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BME 130 – Genomes Lecture 19 The histone code
Figure 7.1 Genomes 3 (© Garland Science 2007)
Figure 10.13 Genomes 3 (© Garland Science 2007) H3 H4 H2A H2B
CHiP-Seq DNA DNA-binding proteins (histones, e.g) crosslink Immuno-precipitate and fragment DNA Immuno-precipitate and fragment DNA Reverse cross-links and sequence
ChIP-Seq and ChIP-chip comparison of two histone marks Lower background & tighter peaks in ChIP-Seq (better contrast) trxG (activation) PcG (repression)
Promoter classes high CpG gene low CpG intermediate CpG CpG Highly expressed, housekeeping genes; other genes N=11,410 Genes with high tissue-specificity N=3,014 Mixture of genes N=3,338
NPC=neural progenitor cellsMEF=embryonic fibroblastsES = embryonic stem cells
Highly dynamic histone modification during differentiation
H3K36me3 marks gene bodies (may prevent aberrant transcriptional initiation)
Imprinting is reflected in H3K36me3 state
Nucleosome positioning and gene structure
Histone modification in cell division Haspin H3 P P Survivin Chromosomal passenger complex AuroraB (kinase)
Section D: Chromosome StructureYang Xu, College of Life Sciences Section D Prokaryotic and Eukaryotic Chromosome Structure D1 Prokaryotic Chromosome Structure.
Epigenetics Xiaole Shirley Liu STAT115, STAT215, BIO298, BIST520.
Figure 7.1 Genomes 3 (© Garland Science 2007) Figure 7.2 Genomes 3 (© Garland Science 2007)
Sequencing Genomes 1) Map the genome 2) Prepare an AC library 3) Order the library 4) Subdivide each AC into lambda contigs 5) Subdivide each lambda into.
Brian P. O’Connor Center for Genes, Environment & Health National Jewish Health Regulation of Cell Identity by a Changing Epigenetic Landscape.
Xiaole Shirley Liu STAT115, STAT215, BIO298, BIST520
20,000 GENES IN HUMAN GENOME; WHAT WOULD HAPPEN IF ALL THESE GENES WERE EXPRESSED IN EVERY CELL IN YOUR BODY? WHAT WOULD HAPPEN IF THEY WERE EXPRESSED.
Understanding the Human Genome: Lessons from the ENCODE project
Lecture #8Date _________ n Chapter 19~ The Organization and Control of Eukaryotic Genomes.
Organization of DNA Within a Cell from Lodish et al., Molecular Cell Biology, 6 th ed. Fig meters of DNA is packed into a 10 m diameter cell.
BME 130 – Genomes Lecture 3 Sequencing technology I The bad old days.
BME 130 – Genomes Lecture 7 Genome Annotation I – Gene finding & function predictions.
13 and 15 September, 2006 Chapter 7 RNA and Chromosome Structure.
Transcriptional profiling I – microarrays and proteomics
D. Cell Specialization: Regulation of Transcription Cell specialization in multicellular organisms results from differential gene expression.
Hybridization Diagnostic tools Nucleic acid Basics PCR Electrophoresis
Genome structures. Table 7.2 Genomes 3 (© Garland Science 2007)
Outline Nucleosome distribution Chromatin modification patterns Mechanisms of chromatin modifications Biological roles.
Regulation of Gene Expression Eukaryotes
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