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PLS221 Capsicum Foto del: The Chile Pepper Institute, NMSU, http://spectre.nmsu.edu/dept/welcome.html ?t=CHILE
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Spooner et al 2008
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C. annuum C. baccatum C. pubescens C. frutescens C. chinense Origin & domestication
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m C. annuum (var. glabriusculum) chacoense galapagence
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Moscone at al 2007 X=13
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Specific traits
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C. annuum C. chinense C. frutescens The Chile Pepper Institute, NMSU, http://spectre.nmsu.edu/dept/welcome. html?t=CHILE
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C. baccatum
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C. pubescens Rocoto, locoto, chile manzano
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Wild species related to C. pubescens C. eximium & C. cardenasii
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C. cardenasii
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C. tovari C. spp Wild species
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Capsicum galapagoense
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Genetic Diversity of Chiltepins
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Variety Clasificacion Non-pungent (sweet): Bell pepper, pimento, paprika, yellow wax 2x acreage than hot peppers Hot peppers (pungent): NewMex type (‘Anaheim’, ‘Largo Verde’), jalapeño, ancho, serrano, Tabasco, habanero etc Paprika in USA is a product: chile powder from non- pungent NewMex types In Europe it is a variety, in Hungary are sweet and hot paprika Bosland et al. 1996. Capsicum pepper varieties and classification
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Bosland et al. 1996. Capsicum pepper varieties and classification
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Genes controlling main traits of Capsicums > 292 described genes for: Morphology,Physiology,Sterility Disease and pest resistance Wang D & P Bosland (2006) HortScience 41:1169-1187
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Pepper Trisomics
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Paran et al Mol. Breed. 13:251-61 Capsicum linkage maps, 2262 markers
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Plant height: dwafs: dw-1, dw-2, dw-3, dw-4, dw-5, dw-6, dw-7, dw-8 Tall: tal Leaf shape : ~31 genes Flowers: Multiple Flowers: Mf-1, Mf-2, Mf-3 Fruit shape: 6 genes: O round; up-1, up2, upright; pf parthenocarpy Immature fruit color: sw1 sulfur white; sw2 yellow-green; sw3 light green, A & MoA, F purple Sample of important genes
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y cl c1 c2 Y Cl C1 C2 red Y Cl c1 C2 light rrd Y Cl C1 c2 orange Y Cl c1 c2 light orange Y cl C1 C2 chocolate y Cl C1 C2 yellow y Cl c1 C2 light yellow y Cl C1 c2 lemon yellow y Cl c1 c2 white y cl - - olive green Mature fruit color
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Y (Ccs) capsanthin y capsorubin synthase, y= zeaxanthin chrom 6 C2: (phytoene synthase), chrom 4 Molecular basis cl: clorophyll retention Unknown function chrom 1
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Heat or Pungencycapsaicinoid alkaloids Most abundant: Heat or Pungency: capsaicinoid alkaloids Most abundant: Capsaicin & Dihydrocapsaicin
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TypespeciesScoville units HabaneroC. chinense210,000 TabascoC. frutescens120,000 Thai HotC. annuum60,000 JalapeñoC. annuum25,000 AmarilloC. baccatum17,000 SerranoC. annuum4,000 BellC. annuum0 Guiness record: Bhut Jolokia de la India, con 1,001,304 US, http://www.nmsu.edu/~ucomm/Releases/2007/february/hottest_chile.htm
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Steward et al. Plant J. 2005 Capsaicinoid synthesis
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The Known Capsaicinoids *Capsaicin * Homocapsaicin *Norcapsaicin Nornorcapsaicin Bis-homocapsaicin Tris-homocapsaicin Tetra-homocapsaicin
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The Known Capsaicinoids *Dihydrocapsaicin * Homodihydrocapsaicin Nordihydrocapsaicin Nornordihydrocapsaicin 3-nor-dihydrocapsaicin Tris-homodihydrocapsaicin Bis-homodihydrocapsaicin Tetra-homodihydrocapsaicin
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The Known Capsaicinoids Isomers of Dihydrocapsaicin Tetra-homodihydrocapsaicin Tris-homodihydrocapsaicin Nordihydrocapsaicin Nornordihydrocapsaicin
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SB2-66, candidate gene Pun1 Stewart et al., 2005, The Plant J. 42:675-688 SB2-66 sequence co- localized with Pun1 in F2 mapping pop C. frutescens BG 2816 (Pun1/Pun1) x C. annum ‘Maor’ (pun1/pun1) Mapped on chromosome 2 DNA seq variation between sween and hot varieties
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Genomic DNA Sequence and structure of Pun1 Genomic DNA Sequence and structure of Pun1 locus Acyltransferase Deletion of 25Kb in sweet types (recessive) of C. annuum
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6 QTL control amount of capsaicinoids Ben-Chaim et al
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Fruit shape and size Zygier et al., 2005 Theor Appl Genet 111:437-445 Ben Chaim et al. 2003b Theor Appl. Genet 106:889-894
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Conservation of QTL on chrom 2 & 4 in Capsicum (3 species) & tomato
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QTL for fruit size (3) fw2.1 (major effect), fw-4.1 y fw4.2 Same locations as tomato QTL for fruit shape (6) fs3.1 y fs10.1 (major effects) not conserved in tomato fs2.1 (minor effect), associated to fw2.1 fs4.1 y fs4.2 (minor effects) associated with fw.4.1 & fw4.2 respectively. fs8.1 (minor effect)
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Nematode resistence (Meloidogyne spp) Djian-Caporalino et al 2007. Theor Appl Genet 114:473-486 6 resistance genes on chrom 9 Me1, Me3 & Me 7 wide spectrum, plus N
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Marker assisted selection for nematode resistance
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Virus Resistence TMV: gene L (or Tm), multiple alleles CMV: gene cm, plus 7 QTL restrict virus movement Caranta et al 2002 Theor Appl Genet 104:586-591 Potyvirus (PVY, TEV Pep-moV, PVMV, CVMV, PVE) Genes prv-1, prv-3,prv-5, pvr-6, prv8 (recessive), Prv-4, Pvr-7, Pn-1 (dominant) Markers available for prv-1, chromos 3 Yeam et al 2005. Theor Appl Genet 112:178-86 TSWS (tomato spotted wilt topovirus) Gene Tsw, marker available, chrom 10
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Resistance to other diseases Bacterial spot (Xanthomonas) Genes Bs-1, Bs-2, Bs-3, Bs-4, Xcv (dominant) Bs-5, bs-6, gds (recessive) Markers available for Bs-2,Bs-3 Phytophtora Gene Psr, Pfr 6 QTL: Phyto4.1,5.1, 5.2, 6.1, 11.1, 12.1 Antracnosis: genes Anr-1 al -5. Oidium: genes lmr-1 al 3, + 7 QTL
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Cytoplasmic male sterility Gulyas et al 2006. Breeding Sci 56:331-4 C. annuum rf in cytoplasm S Restorer Rf Markers distinguishing sterile and fertile plants
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Pepper cms Kim & Kim 2005
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Fruit taste and aroma Very little know on inheritance of compounds determining these traits Specific flavor Bell-pepper (2-isobutyl 3 methoxypyrazine, decadienal) habanero, jalapeño, baccatum yellow pepper? habanero, jalapeño, baccatum yellow pepper? Species specific? Changes during fruit maturation?
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baccatum (yellow) chinense (habanero) annuum (jalapeño) Negre & Quiros,unpub
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#Compound Jalapeño (C. annuum) Habanero (C. chinense) Amarillo (C. baccatum) 1Hexyl 2-methylbutanoate xxx 2 -himachalene xx 33-methylbutyl 3-methylbutanoate xx 4Hexyl 2-methylpropanoate xx 5cis-3-hexenyl 3-methylbutanoate xx 6Hexyl 3-methylbutanoate xx 7Hexylhexanoate xx 8 -cubebene xx 9 cis- -ocimene x 10Isocaryophyllene x 11 -himachalene x 124-methylpentanol x 13cis-3-hexenol x 14Hexanol x 15Heptanol x 165-methyl 6-heptenol x 17Pentyl 3-methylbutanoate x 183,5-dimethylcyclohexene x 192,2-dimethylheptylpropanoate x 202-methylheptylbutanoate x 213,3-dimethylcyclohexanol x 22Octyl 3-methylbutanoate x 23cis-5-octenol x 24 -ionone x + 12 others specific to yellow
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Available to Capsicum breeder Allows to obtain homozygous lines, including RI. Twin seedlings (traditional) Anther culture Doubled Haploides
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Transgenesis Agrobacterium transformation difficult in Capsicum annuumDificulties: 1) Shoot regeneration is genotype dependent 2) Low efficiency of infection by Agrobacteriumin cot/hypocots 2) Low efficiency of infection by Agrobacterium in cot/hypocots Virus resistence obtained experimentally Lee et al (2004) Plant Cell Rept 23:50-58
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Celestial smile The Chile Pepper Institute, NMSU, http://spectre.nmsu.edu/dept/welcome.html?t=CHILE
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