1. The Lionfish Tutorials Dissection

2. Lionfish in the Atlantic

4. Goals of Dissection Determine status and assess changes in: –Physiology –Biology –Ecology

5. More specifically… Condition Population dynamics Growth and Age Reproduction Feeding ecology Genetics

6. Lionfish Dissection Data collected: -Total & standard length -Weight -Sex & gonad tissue sample (reproductive status) -Gill tissue/fin clips (DNA analysis) -Tissue sample (stable isotope analysis) - Otoliths (growth) - Gape height & width - Stomach contents

7. http://216.218.184.99/lionfish/lionfish_dev/sites/default/files/documents/Lionfish%20Dissection.pdf

8. Euthanasia and Animal Welfare Standards for humane treatment of animals used in scientific study Euthanization of collected animals –Eugenol –Ice Slurry –Pithing

9. Handling precautions and procedures Venomous spines Precautions Treatment Disposal of specimens

10. Measurement and Sampling External Internal Units Minimizing error Data sheets

11. Dissection Methodology External –Length –Weight –Gape –Tissue

14. Internal Sampling Opening the gut cavity Gender ID and reproductive staging Fat Stomach Otoliths

18. Gonad Staging Male - Gonads elongated with ratio of length:width greater than 2 M1 – Immature – Testes appear as clear, threadlike structures 1-3 mm in diameter and 5-10 mm in length. Immature female ovaries are largely indistinguishable from immature male testes. M2 – Mature – Testes appear cream-colored with well-defined edges. Testes are typically not larger than 10 mm in diameter and 20 mm in length in the largest of specimens.

19. Gonad Staging Female - Gonads oval, cream-pink in color, ratio of length:width less than 2. F1 – Early Developing - Ovaries are cream colored and round with no edges. Width may vary from 5 - 15 mm. Eggs are not visible macroscopically. Ovaries are more firm than during the Developing stage. F2 – Developing – Ovaries are cream colored with some pinkish portions. Eggs are visible as small white spheres. Ovaries may vary from a width of 15 mm to more than 30 mm. No gelatinous mucus is visible around periphery.

20. Gonad Staging Female - Gonads oval, cream-pink in color, ratio of length:width less than 2. F3 – Mature/Spawning Capable- Ovaries are large with clear gelatinous mucus containing visible eggs. Ovary size may vary from a width of 15 mm to more than 30 mm. F4 – Actively Spawning – Ovaries are hydrated. A large number of clear eggs are encompassed in gelatinous mucus visible along periphery of the ovary.

21. Lionfish Fat

22. Nassau grouper Yellow tail snapper Banded coral shrimp Lionfish consume prey over half their size Reef squirrelfish Spanish hogfish Redband parrotfish Over 70 different prey species found in lionfish stomachs Commercially and recreationally important species (grazers and cleaners) – keeping the reef healthy and fish clean

23. 64 prey fish plus a shrimp!

24. Stomach Contents

25. Lionfish Stomach Contents For each prey: -Identification -Total length -Digestion level -Scale of 1 to 5: 1= ID to species level, 5= unidentifiable) - Volume

26. Otolith removal

27. WWW.REEF.ORG WWW.NOAA.GOV WWW.GCFI.ORG

28. WWW.REEF.ORG WWW.NOAA.GOV WWW.GCFI.ORG Acknowledgements Dr Stephanie Green Dr James Morris Lad Akins Dr Dayne Buddo Cape Eleuthera Institute REEF