1. Repressilator: “I’ll be back.” http://www.freefoto.com/images/11/11/11_11_12---Portland-Bill-Lighthouse_web.jpg

2. Repressilator: “I’ll be back.” Is it possible to get a culture of E. coli cells to flash on and off with a green light? A Synthetic Oscillatory Network of Transcriptional Regulators; Michael B. Elowitz and Stanislas Leibler; Nature. 2000 Jan 20;403(6767):335-8.

5. Repressilator: “I’ll be back.” Is it possible to get a culture of E. coli cells to flash on and off with a green light? YES!!

6. Clonal cells = clonal action?

7. Repressilator: “I’ll be back.” Is it possible to get a culture of E. coli cells to flash on and off with a green light? NO!!

8. Repressilator: “I’ll be back.” What worked and what did not? How could you regulate the repressilator to be synchronous?

9. Alternative Representations What if you don’t understand the molecular biology short hand, can this information be presented in a different way to reach a wider audience?

13. Repressilator: “I’mmmm back.” Could you assemble a repressilator from basic DNA parts using the BioBrick assembly method?

14. Registry of Standard Biological Parts Searchable database of parts Physical collection of cloned parts Growing with each iGEM competition Parts exist as BioBricks –Standardized assembly –Parts, intermediates, devices, machines More characterization needed Mistakes in Registry exist

16. How to Search the Registry Type a term into the Search field on the main Registry page (good luck with that) Click on “Browse Parts by Type” Click on Part Searches –Search with a phrase of text –Search for subparts of a composite part –Search for superparts that contain a part

20. Useable Parts Once you find a part, navigate to its “Part Main Page” Make sure it is listed as “DNA Available” in the upper right corner Click on “Physical DNA” and find the Library location at the bottom of the page