1. The Peritoneal Vac Study How do I enrol a patient in this study? PI: Andrew Kirkpatrick RC: Corina Tiruta

2. 1.Decide if an open abdomen is the indicated therapy for your patient. If so, continue to step 2; If not, do not enrol this patient into this study. Exclude: patients under 18 years old, pregnancies, patients undergoing intraperitoneal chemotherapy 1.Go to www.peritonealvac.com 2.Select Randomization hot-key (top right corner) 3.Enter the super-simple Username and Password (if forget can page Andy #02603, 24/7) 4.Enter patient RHRN + last name to randomize 5.Youll be indicated the patient number you have randomized. Use the corresponding bag (located in the AbThera dressing tote, in the Peritoneal Vac Study box) for blood & peritoneal fluid collection. e.g.: for randomized pt.1 use the kit labelled with pt.id 001-1

3. 7.Collect PERITONEAL FLUID: -If Mon. to Fri., 8am to 4pm: fill-up one 50ml blue cap tube and one 4ml green cap tube labelled PF with peritoneal fluid, and page Caroline Leger at 07559 to collect the 50 ml tube. -If after hours: fill-up one 4ml green cap tube labelled PF with peritoneal fluid; discard the 50ml tube. 8.Collect BLOOD If Mon. to Fri.8am to 4pm or after hours: -2 Green Cap (4ml) tubes for plasma isolation (labeled with a P) -2 Red Cap (4ml) tubes for serum isolation (labeled with a S) fill up the tubes to 4ml and keep them on the rack provided in an upright position * blood will be drawn by anaesthesia team

4. 9.Fill-out the pt. gender & DOB, collection date&time on the Research Requisition 10.Send the five (4ml) tubes, the rest of the bag (containing 3 empty blue cap 15ml tubes for CLS use only) and the Research Requisition to CLS by porter 11.Place the type of Temporary Abdominal Dressing indicated by randomization - for further information on actually dressing placement refer to Regional Trauma Services Guidelines for the Mgmt.of the Open Abdomen (internal website under Trauma Services/Adult Trauma Care/Policies,Protocols and Practice Guidelines) or page Andy #02603 24/7

5. STEP 1: Peritoneal Fluid Collection: Fill with Blood from arterial line or venipuncture and keep upright in the rack STEP 2: Blood Collection: STEP 3: Fill the requisition STEP 4: Calgary Lab Services: Call a porter to have the 5 tubes (green and red cap) + the research requisition and the study bag containing 3 unfilled 15ml transfer tubes delivered to CLS Fill BOTH with Peritoneal Fluid Dont send the 50ml tube to CLS. page Caroline at 07559 to pick it up Monday to Friday 8am to 4pm: PF

6. Fill with Peritoneal Fluid STEP 1: Peritoneal Fluid Collection: PF Fill with Blood from arterial line or venupuncture and keep upright in the rack STEP 2: Blood Collection: STEP 3: Fill the requisition STEP 4: Calgary Lab Services: Call a porter to have the 5 tubes + the research requisition and the study bag containing 3 unfilled transfer tubes delivered to CLS Monday to Friday after 4pm, week-ends and holidays