1. The 6420 Triple Quad LC/MS System
Routine, Robust and Reliable Quantitation 1

2. Agilent has the Newest Fleet of LC Triple Quads
A New Agilent LC Triple Quad
Launched
Every Yr
For last
4 yrs
6460
Triple
Quad
6430 Triple Quad
6420 Triple Quad
6490
6460 Triple Quad
Performance 2

3. 6420 Enhancements over Earlier 6410
New Capillary front end: faster polarity switching better ion transmission
New electronics, faster MRMs
New Agilent rough pump
Upgradeable to a 6460 with Agilent Jet Stream Technology
6420 Triple Quad LC/MS
Waters requires a 10 msec interscan delay to achieve zero cross-talk.
This argument has been successfully used in a few sales situations.

4. 6420: Fast Electronics for High Sample Throughput
The Agilent 6420 Triple Quad features fast and rugged electronics;
Making it a workhorse for high throughput labs
Fast MRMs , dwell time of: 1 msec
Fast polarity switching : 30 msec
Fast Triggered MRM
(for ten ion transitions): msec

5. Future Proof Your LC Triple Quad Investment
Infinity LC
6420
Routine
Analysis
Ultra-Trace Analysis
6430
Performance
Trace
Step 1
Step 2
6460

6. 6420 Upgrade Possibilities
S/N Spec
Product
Upgrade
Options
Installed
in your
Lab
6430
to 6460
Agilent
Jet Stream
& High Mass Quads
Yes
6420
to 6430
Second
Turbo 8X
6460
Triple
Quad
6430 Triple Quad 2X
6420 Triple Quad 1X

7. New 6420 LC Triple Quad Supports:
Agilent Upgrade Kits to 6430 and 6460 LC Triple Quads
Agilent Dynamic MRM
Agilent Fast Track Kits and Data Bases
Agilent Triggered MRM

8. Upgradeable by Design – 6400 Upgrades
6420 to 6430 Upgrade
Improves Signal-to-Noise up to two-fold for some compounds
The 6420 is preconfigured to accept a second turbo pump for enhanced sensitivity.
Higher vacuum in Q3 reduces noise and allows greater ion conductance in the collision cell.

9. 6430 to 6460 Add: Agilent Jet Stream Technology thermal confinement of ESI spray cone
6430 to 6460 Upgrade
Sensitivity Spec: 4X higher
25 0C
350 0C after 8 min

10. 6430 to 6460 Upgrade: High Mass Quads – 3K
The 6460 mass range is 3,000 m/z:
Pegylated peptides
Acylated peptides
Lipopolysaccharides
Glycans
Natural Products

11. Dynamic MRM groups MRMs in Retention Windows
Dynamic MRM creates powerful quantitative methods by grouping MRMs in retention time windows instead of time segments.
Dynamic MRM methods are created by importing compound specific MRMs and retention times from a Fast Track Data Base or worksheet
Dynamic MRM supports up to 4,000 MRMS for all 6400 Series Triple Quads
Dynamic MRM also supports polarity switching

12. Dynamic MRM for the new 6420 Triple Quad1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
Retention Time Windows with overlapping MRMs

13. Agilent Fast Track Kit for Forensics
Includes a Dynamic MRM method,
200 compound data base with
optimal settings and RTs
FORENSIC/TOXICOLOGY TEST MIX – 24 compounds
Compound Name Precursor Ion Product Ion Collision Energy Ret Time
Hydrocodone methamphetamine
MDMA
Strychnine
MDEA ……

14. Forensics Test Mixture on the new 6420
25 pg of each on-column
Acquisition Time (min) 1
1.2
1.4
1.6
1.8 2
2.2
2.4
2.6
2.8 3
3.2
3.4
3.6
3.8 4
4.2
Amphetamine
Metham-phetamine
Trazodone
PCP
Norver-apamil
Verapamil
Methadone
Proadifen
Benzoyl-ecgonine
Cocaine

15. Triggered MRM (tMRM) – a Data Dependent Scan
Data dependent execution of additional, confirmatory MRMs
152.1
77.0
105.0
219.0
m/z 60
100
140
180
220
209.1
165.1
triggered MRM Spectra
2-Ethylanthraquinone

16. Triggered MRM in MassHunter Acquisition
Triggered cycle (above threshold)
Additional MRMs used
for compound confirmation
Threshold
Primary cycle (below threshold)

17. tMRM Product Ion Spectrum on any 6400 Triple A Re-Constructed Product Ion Spectrum that is Library Searchable
334 > 76
334 > 91
334 > 105
334 > 117
334 > 119
x103
x103
x103
x103
x103 8 7 6 5 4 3 2 1 8 7 6 5 4 3 2 1 8 7 6 5 4 3 2 1 8 7 6 5 4 3 2 1
117.0 8 7 6 5 4 3 2 1
76.0
91.1
105.1
119.0 80
145.0
334 > 171
x103
334 > 132
x103
334 > 145
x103
334 > 147
x103 8 7 6 5 4 3 2 1 8 7 6 5 4 3 2 1 8 7 6 5 4 3 2 1 8 7 6 5 4 3 2 1
147.0
132.1
171.1
tMRM Product Ion Spectrum
Tebufenpyrad
x103
145.0
117.0 8 7 6 5 4 3 2 1
This is an illustration of how tMRM acquisition generates the product ion spectrum for each triggered analyte.
Up to ten MRM transitions are acquired for each target (the primary transitions are typically the transitions with the highest product ion abundance).
The tMRM software then compiles the primary and secondary MRM spectra into a single, library searchable, product ion spectrum
With up to ten product ions present in each tMRM product ion spectrum for each target analyte, there is sufficient data to confidently identify or confirm the analyte of interest with library confirmation. The biggest advantage of using tMRM to generate these product ion spectra (rather than conventional product ion scanning instruments), is that acquiring all ten of the MRM transitions which make up this spectrum takes about 20 ms, where a typical product ion scan on a trap or TOF instrument takes about 200 ms. The faster cycle time for qualitative analysis using tMRM acquisition means that more data points are acquired across each peak, and the quantitative data acquired is more sensitive, robust, and accurate.
76.0
91.1
105.1
132.1
171.1
334.2

18. Benefits of triggered MRM
More than ten times more sensitive than full scan MS/MS data
Searchable with NIST MS/MS 2.0 Library
Searchable with Agilent Accurate Mass MS/MS Library
Compatible with custom, user created libraries
MassHunter automatically creates custom triggered MRM library from standards

19. Forensics Mixture with 6420 and tMRM Method
Triggered MRM actuated on red peak
Acquisition Time (min) 1
1.2
1.4
1.6
1.8 2
2.2
2.4
2.6
2.8 3
3.2
3.4
3.6
3.8 4
4.2
Amphetamine
Metham-phetamine
Trazodone
PCP
Norver-apamil
Verapamil
Methadone
Proadifen
Benzoyl-ecgonine
Cocaine

20. Triggered MRM for Compound Confirmation
6420 tMRM: Benzoylecgonine, 25 pg on column
Mass-to-Charge (m/z)
100
150
200
250
300
-1.2 -1
-0.8
-0.6
-0.4
-0.2
0.2
0.4
0.6
0.8 1
168.1
77.0
105.0
290.1
Ref. Library Match Score = 95.1
Peak Product Ion Spectra
Library Reference
Product Ion
Spectra
@ 5ppb level:
RSD=4.9% (N=3)

21. 6420 Summary
DENVER (American Society for Mass Spectrometry conference), June 6, 2011
The new Agilent 6420 Triple Quad LC/MS System is a workhorse triple quad designed to be readily upgradable to the higher-performance 6430 and 6460 instruments.
The new 6420 is more productive than the previous 6410 model with faster electronics and new software features. Our popular dynamic MRM solution for grouping ion transitions by retention time has been enhanced with the addition of triggered MRM for simultaneous compound confirmation.
The Agilent 6420 is well suited for both routine and complex analyses such as toxicology and drugs of abuse testing. It is designed for round-the-clock operation to support labs running a large number of samples.
The 6420 replaces Agilent's popular Model 6410 triple quad. The 6420 maintains method and data compatibility with existing 6410 methods. The 6420 can be easily incorporated into high-productivity laboratories with multiple 6410 systems.

22. THANK YOU! 22