1. Image J Sample Pictures Available at http://biology2020.wikispaces.com/

2. Onion Find your file and open it.

3. Select the straight line tool. Drag across your field of view.

4. Under analyze: Set scale

5. Pixels are already there. Enter your known distance.

6. Trace a cell wall with the free hand tool (before).

7. Go to analyze: Measure

8. Analyze: Set Measurements

10. Measure DNA Distance

11. Leaf Surface Area

12. Compare Stomata per surface area Oak 400XRose 400X

13. Choose the Multi point tool (right click)

14. Click to number (21 in the field of view)

15. Diameter= 0.45 mm Radius= 0.225 mm Area of FOV= 0.145mm 2 (from Image J) Verify ?