2. Introduction
Biochemize S.L. is a biotechnological company focused in the design and development of processes based on biocatalysis.
Our technological platform lies in the use of enzymes or microorganisms to perform chemical reactions able to substitute manufacturing processes based on classical organic chemistry.

3. Location We are located in Terrassa, at 20 km from Barcelona.
Our office and laboratories are part of the facilities of the Technology Centre of our associate partner Leitat.

4. Our Technology
Biochemize S.L. develops bioprocesses using biocatalysts (enzymes or microorganisms) in single or multiple transformation steps.
Our technological platform P2B (Platform to Biochemize) is capable to perform high throughput screening of biocatalysts, reaction conditions, suitable substrates and products obtained.
The screening can be done in a short period of time and renders information about different possible bioprocesses and conditions that could be further developed at a larger scale.

5. P2B – Platform To Biochemize
P2B is the key to determine quickly with low risk and at very small scale if a conceptual biotransformation is technologically viable.
Once the succeeding biocatalysts or bioprocesses have been identified, Biochemize S.L. investigates at lab scale the experimental conditions which can lead to the highest yields.
Two approaches can be followed:
P2B Bioprocess
Development of bioprocesses suitable to substitute already established synthetic procedures up to an industrial scale
P2B Discovery
Perform biotransformations that can lead to a (new) chemical entity with improved features compared to the starting molecule

6. P2B – Discovery
P2B Discovery is an ideal approach for molecules with a complex structure or different functional groups that can be affected when trying to modify them by synthetic procedures.
Biocatalysts can be used to obtain new lead, lead optimization, me-too and me-better compounds by modification of already available structures.

7. P2B – Discovery The biotransformation can be: Directed
The type of transformation is selected based on the functional group or structure targeted.
This approach is suitable when it is well known what feature is conferred by the new functional group or structure in the final molecule (e.g. make it more soluble)
Exploratory
The transformations applicable are diverse in order
To test what kind of molecules can be obtained from
a given starting structure or what kind of final properties may be achieved.
This approach is appropriate to seek new development opportunities (e.g. improved or different pharmacological properties)

8. P2B – Discovery Biotransformations
Oxidations / Reductions
Hydration / Dehydration
Glycosilations
Sulfidations / Sulfoxidations
Aromatic Chlorination
N-, O-, S-methylation
Nitrilation
Alkylation / Dealkylation
Amide and Ester formation / hydrolysis
Potentially, any biotransformation already described…

9. Applications of P2B – Discovery
Evaluation of pharmacological properties
Improve the solubility
Source of building blocks
Obtain more stable molecules
Increase the IP of the company or the pipeline of products
Support in Analytical Methods development
More…

10. Project Management in P2B – Discovery
The P2B Discovery process consists of the following phases:
Bibliography
Assessment
Biotransform.
Screening
Biocatalyst
Selection &
Reproduction
Compound
Characteriz.
Small Scale
Production
Tests of
Properties
by Customer
PHASE I
PHASE II
PHASE III
PHASE IV
PHASE V
Flexible: Phases can be omitted, merged, delimited or adapted to each project or customer requirements.
Step-wise: Each phase is not planned nor quoted until the previous phase is completed and the results presented.
Risk-Control: The customer decides the project continuation at each phase depending on results and conclusions obtained so far.
Fast: The objective is to provide small amounts of promising (new) molecules in a short time, avoiding setting-up long, difficult and low yield synthetic procedures.
Results oriented: The customer is able to decide if full development is a business opportunity according to the properties tests of molecules provided.

11. Operations
Biochemize S.L. offers the most outstanding and up to date technology options, while maintaining an industrial scope.
If necessary, many operations can be outsourced to other companies and research centres under our direction and supervision.
We have a powerful network of contacts in biocatalysis and bioprocessing. Several partnership agreements have been established.
Advisors
Analytical Services
Biocatalyst Suppliers
Bioprocessing Services
Biotransformation Experts
Enzyme Screening
Enzyme Immobilization
Genetic Engineering
Manufacturers
Research & Development
Scaling-Up Services
Austria
Bulgaria
China
Denmark
Ireland
Italy
Korea
Netherlands
Spain
United Kingdom
United States

12. Why contracting Biochemize?
We offer the most outstanding and up to date technology options.
Industrial scope guaranteed: productivity, costs and risks are the key drivers of our evaluations.
Our technology platform (P2B) is a powerful tool for fast, low risk and small scale proof of concept verifications.
We increase the success possibilities through the P2B platform and our network of contacts.
Project management based on a development by phases approach.
The customer controls the risks and costs associated to the project development.

13. Contact Jaume Mir Founder CEO and Commercial Director
Telephone:
Address: C/. de la Innovació, 2
(Leitat Centre Tecnològic)
88225 – Terrassa
(Barcelona) SPAIN

15. Applications of P2B – Discovery
Back-up slides
Applications of P2B – Discovery

16. Applications of P2B – Discovery
Evaluation of pharmacological properties
Changes in the structure of the original molecule can influence the pharmacological properties:
More specific action (i.e. less side effects)
Increase the potency
New activity

17. Applications of P2B – Discovery
Improve the solubility
A small change in the structure of a Drug Substance can modify the solubility without affecting the pharmacological activity.
The solubility can be modified by introducing polar or hydrophobic groups, as needed.
Possible applications: better formulability during the pharmaceutical processing, improvement of the ADME profile.
R= OH, Cl

18. Applications of P2B – Discovery
Source of building blocks
The cleavage of some functional groups or parts of the molecule can lead to a building block that can be further modified by synthetic or biotechnology approaches.
This option is specially useful in compounds with a (poly)glycosyl group, that can be removed to render the aglycone part.

19. Applications of P2B – Discovery
Obtain more stable molecules
Physical, chemical and microbiological stability can be improved by the new properties conferred by the structural changes induced in the original molecule:
Physical: crystalline form, particle morphology
Chemical: hydrophobization, steric hindrance
Microbiological: prevention of enzyme attack, 3D conformation, anti-microbial activity

20. Applications of P2B – Discovery
Increase the IP of the company or the pipeline of products
Providing new molecules that can be potentially developed, licensed or marketed can increase the value of your company and open new business opportunities.

21. Applications of P2B – Discovery
Support in Analytical Methods development
Obtain metabolites or impurities difficult to synthesize, but necessary to develop suitable control testing procedures.
The technical information in your DMF and dossier submissions will be strongly supported with a solid characterization package.
If you are a Drug Substance manufacturer, you can give support to your customers in their Drug Product development, by supplying these special molecules.

22. Project Management in P2B – Discovery
Back-up slides
Project Management in P2B – Discovery

23. Project Management in P2B – Discovery
PHASE I
Biocatalyst
Selection &
Reproduction
Tests of
Properties
by Customer
Bibliography
Assessment
Biotransform.
Screening
Compound
Characteriz.
Small Scale
Production
PHASE I
PHASE II
PHASE III
PHASE IV
PHASE V
The Bibliography Assessment comprises preliminary evaluations, some of them optional, that can be performed by Biochemize or by the customer.
The scope of the assessment includes:
Theoretical evaluation of suitable biotransformations according to the original structure (Biochemize)
Qualitative estimation of possible structure-activity relationships (QSAR) in order to select appropriate biotransformations (optional)
Verification of intellectual property opportunities and already protected structures (optional)

24. Project Management in P2B – Discovery
PHASE II
Biocatalyst
Selection &
Reproduction
Tests of
Properties
by Customer
Bibliography
Assessment
Biotransform.
Screening
Compound
Characteriz.
Small Scale
Production
PHASE I
PHASE II
PHASE III
PHASE IV
PHASE V
The biotransformations selected in the Phase I evaluation are confirmed or discarded during the Phase II with an Experimental Screening.
The aim of this phase is to afford evidence that the substrate is transformed and what kind of general biotransformations occur.
The final product isolation is not possible in this phase, because of the very small scale experiments. Thus, the characterization is not attained, although some hints about the resulting structure may be obtained.

25. Project Management in P2B – Discovery
PHASE III
Biocatalyst
Selection &
Reproduction
Tests of
Properties
by Customer
Bibliography
Assessment
Biotransform.
Screening
Compound
Characteriz.
Small Scale
Production
PHASE I
PHASE II
PHASE III
PHASE IV
PHASE V
A selection of the biotransformations and the corresponding biocatalysts is done among those succeeding in Phase II.
The experiments are reproduced in different experimental conditions in order to meet the requirements leading to a higher productivity at a laboratory scale.
The aim at this stage is to obtain enough amount of the resulting product isolated for its characterization in the next phase.

26. Project Management in P2B – Discovery
PHASE IV
Biocatalyst
Selection &
Reproduction
Tests of
Properties
by Customer
Bibliography
Assessment
Biotransform.
Screening
Compound
Characteriz.
Small Scale
Production
PHASE I
PHASE II
PHASE III
PHASE IV
PHASE V
The compound characterization is an optional phase.
It can be led by Biochemize or performed by the customer, as required.

27. Project Management in P2B – Discovery
PHASE V
Biocatalyst
Selection &
Reproduction
Tests of
Properties
by Customer
Bibliography
Assessment
Biotransform.
Screening
Compound
Characteriz.
Small Scale
Production
PHASE I
PHASE II
PHASE III
PHASE IV
PHASE V
The final stage of P2B-Discovery is the small scale production of the final product (mg to g) in order to allow the customer to test the properties.
If a full development of the product is finally decided, the manufacturing process at higher scale can be set-up by:
The customer: according to in-house procedures, synthetic chemistry, out-sourcing to contractors or partners, etc.
Biochemize: using the P2B-Bioprocess approach and according to the knowledge gained with the P2B-Discovery experience, or through technology provided by own partners, etc.