1. AUTORADIOGRAPHY

2. Radioisotopes Isotopes(which have same chemical but different physical properties) having radioactivity. Natural occurrence is rare because of its instability. They emit different radiations such as α,β and γ.

3. Detection of disintegration: Electrical Eg: GM counter, Ionisation counter, Gas flow counter Scintillation Eg: scintillation counter Autoradiography

4. WHAT IS AUTORADIOGRAPHY…?

5. Principle Incubate tissue with radioactive ligand Expose to film or emulsion Isotope will emit radiation (usually beta) Radiation will hit silver grains in emulsion and expose them

6. 1867 Uranium salts responsible for blackening of photographic film(Niepce de st.victor) 1896 K 2 UO 2 (SO 4 ) 2 emits radiation affecting a photographic film(Becquerel) 1924 First autoradiogram –distribution of Po in biological material 1943 Autoradiographic demonstration of 131 I in tissue sections of thyroid 1946 Liquid photographic emulsion for autoradiography(Belanger and LeBlond) 1950 ’Stripping film’ technique for microscopic autoradiography 1970 …Numerous biological applications… ~1990 Alternatives to film: Imaging plate technology HISTORY

7. PROCEDURE

8. TYPES: ♦ In-vivo autoradiography ♦ In-vitro autoradiography

9. Cont… MICROSCOPIC AUTORADIOGRAPHY Hypophysis of rat labelled with 3 H

10. Macroscopic autoradiography Whale body autoradiography Localization on chromatogram or gel

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