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AUTORADIOGRAPHY. Radioisotopes Isotopes(which have same chemical but different physical properties) having radioactivity. Natural occurrence is rare because.

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Presentation on theme: "AUTORADIOGRAPHY. Radioisotopes Isotopes(which have same chemical but different physical properties) having radioactivity. Natural occurrence is rare because."— Presentation transcript:

1 AUTORADIOGRAPHY

2 Radioisotopes Isotopes(which have same chemical but different physical properties) having radioactivity. Natural occurrence is rare because of its instability. They emit different radiations such as α,β and γ.

3 Detection of disintegration: Electrical Eg: GM counter, Ionisation counter, Gas flow counter Scintillation Eg: scintillation counter Autoradiography

4 WHAT IS AUTORADIOGRAPHY…?

5 Principle Incubate tissue with radioactive ligand Expose to film or emulsion Isotope will emit radiation (usually beta) Radiation will hit silver grains in emulsion and expose them

6 1867 Uranium salts responsible for blackening of photographic film(Niepce de st.victor) 1896 K 2 UO 2 (SO 4 ) 2 emits radiation affecting a photographic film(Becquerel) 1924 First autoradiogram –distribution of Po in biological material 1943 Autoradiographic demonstration of 131 I in tissue sections of thyroid 1946 Liquid photographic emulsion for autoradiography(Belanger and LeBlond) 1950 ’Stripping film’ technique for microscopic autoradiography 1970 …Numerous biological applications… ~1990 Alternatives to film: Imaging plate technology HISTORY

7 PROCEDURE

8 TYPES: ♦ In-vivo autoradiography ♦ In-vitro autoradiography

9 Cont… MICROSCOPIC AUTORADIOGRAPHY Hypophysis of rat labelled with 3 H

10 Macroscopic autoradiography Whale body autoradiography Localization on chromatogram or gel

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