1. Genome Editing with mRNA Encoding ZFN, TALEN, and Cas9
Hong-Xia Zhang, Ying Zhang, Hao Yin 
Molecular Therapy 
Volume 27, Issue 4, Pages (April 2019)
DOI: /j.ymthe
Copyright © 2019 The American Society of Gene and Cell Therapy Terms and Conditions

2. Figure 1 Genome Editing Mediated by Site-Specific Nucleases
The zinc-finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or CRISPR-Cas genome-editing nucleases are able to quickly search the genome, specifically bind various DNA sequences of interest, and efficiently induce double-strand breaks (DSBs). The DSBs are then effectively repaired by non-homologous end joining (NHEJ), or potentially homology-directed repair (HDR) if provided a DNA donor template, resulting in gene disruption, gene correction, or gene insertion, respectively.
Molecular Therapy  , DOI: ( /j.ymthe )
Copyright © 2019 The American Society of Gene and Cell Therapy Terms and Conditions

3. Figure 2 Barriers for Non-viral Delivery
Systemic delivery of genome-editing nucleases using non-viral vectors has to overcome various barriers.
Molecular Therapy  , DOI: ( /j.ymthe )
Copyright © 2019 The American Society of Gene and Cell Therapy Terms and Conditions