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Fig. 4 mRNA induction by CU-T12-9 and Pam3CSK4 in Raw 264

Published bySabine Baumgartner Modified over 5 years ago

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Presentation on theme: "Fig. 4 mRNA induction by CU-T12-9 and Pam3CSK4 in Raw 264"— Presentation transcript:

1 Fig. 4 mRNA induction by CU-T12-9 and Pam3CSK4 in Raw 264
Fig. 4 mRNA induction by CU-T12-9 and Pam3CSK4 in Raw macrophage cells. mRNA induction by CU-T12-9 and Pam3CSK4 in Raw macrophage cells. (A) CU-T12-9 (1 μM) and 33 nM (50 ng/ml) Pam3CSK4 increased the TLR1 mRNA expression over time. Shown are the changes in relative gene expression levels (fold change) relative to the expression levels in dimethyl sulfoxide (DMSO)–treated controls, represented as means ± SD. *P < 0.05 for Pam3CSK4 or CU-T12-9 relative to DMSO-treated control. The statistical analyses were based on two independent biological replicates, and each biological replicate was divided into three samples for independent measurements. Changes in the relative expression of hTLR1 were standardized to the expression of the housekeeping gene GAPDH (glyceraldehyde-3-phosphate dehydrogenase). (B) Dose-dependent assay of CU-T12-9 triggered TLR1 mRNA after the cells were treated for 24 hours. (C) CU-T12-9 and Pam3CSK4 increased TLR2 mRNA at 2 hours with a gradual decline in TLR2 mRNA expression by 24 hours. *P < 0.01 for Pam3CSK4 or CU-T12-9 relative to DMSO-treated control. (D) Dose-dependent activation with CU-T12-9–induced TLR2 mRNA in 2 hours. (E) TNF mRNA was activated by CU-T12-9 and Pam3CSK4 through the NF-κB pathway, and the highest signaling was seen at 8 hours. *P < 0.01 for Pam3CSK4 or CU-T12-9 relative to DMSO-treated control. (F) CU-T12-9 showed dose-dependent activation of TNF mRNA at 8 hours. (G) Gradual increase in iNOS mRNA expression over time with CU-T12-9 and Pam3CSK4 through the NF-κB pathway compared with vehicle control. **P < for Pam3CSK4 or CU-T12-9 relative to DMSO-treated control. (H) CU-T12-9 showed dose-dependent activation of iNOS mRNA at 24 hours. (I) IL-10 mRNA up-regulated by CU-T12-9 and Pam3CSK4 through the NF-κB pathway in 2 hours, and gradual decrease in IL-10 mRNA in 8 and 24 hours. *P < 0.01 for CU-T12-9 relative to DMSO-treated control. (J) CU-T12-9 showed dose-dependent activation of IL-10 mRNA at 24 hours. Kui Cheng et al. Sci Adv 2015;1:e Copyright © 2015, The Authors

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