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Fig. 1 Design of a FRET reporter for RIG-I activation.

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Presentation on theme: "Fig. 1 Design of a FRET reporter for RIG-I activation."— Presentation transcript:

1 Fig. 1 Design of a FRET reporter for RIG-I activation.
Design of a FRET reporter for RIG-I activation. (A) Donor and acceptor fluorophores were placed on the 2CARD and hel2i domains of RIG-I, respectively. Models of RIG-I in the autoinhibited state [Protein Data Bank (PDB) ID: 4A2W] and the activated state (PDB ID: 5F9H) predict that the distance between fluorophores changes significantly upon activation. (B) FlAsH donor fluorophore was attached by inserting a tetracysteine motif (CCPGCC) immediately C-terminal to the 2CARD domain. Alexa Fluor 594 acceptor fluorophore was attached via copper-free click chemistry to the unnatural amino acid azido-phenylalanine (AzF), which was incorporated by amber codon suppression. Structures were modeled using PyMOL (49). T. H. Dickey et al. Sci Adv 2019;5:eaax3641 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).

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