1. Greater influx of PMNs and ST using PEGPH20/shIDO-ST.
Greater influx of PMNs and ST using PEGPH20/shIDO-ST. A, mice treated with PEGPH20, PEGPH20/shScr-ST, or PEGPH20/shIDO-ST were imaged at indicated time points following start of ST treatment. For each time point, mice were first injected with NS-NIR imaging agent specific to PMNs followed by injection with d-luciferin. Images are representative of at least two separate experiments using identical treatment regimens. B, sections of tumors from mice treated with ST alone, PEGPH20/shScr-ST, or PEGPH20/shIDO-ST were stained for detection of ST, PMNs, and intact nuclei. ST alone represents mice treated with either shScr-ST or shIDO-ST. Scale bar, 1 mm. C, areas of PEGPH20/shIDO-ST–treated tumors were magnified (×100) to visualize colocalization of ST and PMNs in PEGPH20/shIDO-ST–treated tumors. Scale bar, 5 μm. D, sections of tumor from indicated treatment groups were stained for activated (cleaved) CC3, PMNs, and intact nuclei. Scale bar, 1 mm.
Edwin R. Manuel et al. Cancer Immunol Res 2015;3:
©2015 by American Association for Cancer Research