1. C. neoformans encodes a single family 2 DHODH required for wild-type growth.
C. neoformans encodes a single family 2 DHODH required for wild-type growth. (A) Multiple sequence alignment of C. neoformans Ura1 protein sequence (top line) with other eukaryotic family 2 DHODHs including (from top to bottom) Ustilago maydis (DQ869012), Candida albicans (AY230865), Schizosaccharomyces pombe (NM_ ), human (NM001361), and Plasmodium falciparum (AB070244), and the family 1 enzyme from S. cerevisiae (P28272) (bottom line), with only the respective N-terminal regions shown. The first 25 amino acids are important for mitochondrial import and membrane anchorage. The boxes highlight the hydrophobic residues that make up the hydrophobic patches in the family 2 DHODHs. (B) Growth of wild-type, ura1Δ mutant, and complemented strains in rich medium (YPD) or minimal medium with and without uracil supplementation. (C) India ink staining of C. neoformans capsule under inducing conditions in the wild type, ura1Δ mutant, complemented mutant, and ura1Δ strain supplemented with uridine at 24, 48, and 72 h. (D) Mean capsule sizes of wild type, ura1Δ mutant, ura1Δ mutant supplemented with uridine, and complement strain.
Dithi Banerjee et al. mSphere 2016; doi: /mSphere