1. Uses and application in the real world
Bioluminescence
Uses and application in the real world

2. BRET: Bioluminescence Resonance Energy Transfer
BRET is a proximity-based assay where the energy generated by the catalytic degradation of coelenterazine by the enzyme Renilla luciferase (Rluc) (energy donor) is transferred to a green fluorescent protein (GFP) acting as the energy acceptor. The GFP then emits light at its specific emission wavelength.

3. Degradation of coelenterazine by the enzyme Rluc

4. BRET

5. BRET: Bioluminescence Resonance Energy Transfer
BRET can be used to observe protein-protein interaction in living mammalian cells.
It is based on the non-radioactive transfer of energy between a luminescent donor Rluc and a fluorescent acceptor (Green Fluorescent Protein or GFP).
May be used in the future to identify new protein complexes in human.

6. Monitoring of ubiquitination in living cells by BRET
Ubiquitination is descried as the rapid process of post-translational modification present in many aspects of biology involving a covalent attachment of ubiquitin to proteins.

7. Monitoring of ubiquitination in ling cells with BRET
Upon degradation of DeepBlueC,the Rluc fuses to β-arrestin and emits blue light with an emission peak at 395 nm. If the β-arrestin is ubiquitinated, a transfer of energy will occur between Rluc and the GFP2 fused to the N terminus of ubiquitin, resulting in re-emission of light with an emission peak at 510 nm.

8. Gene expression
Bioluminescence can be used to study prokaryotic gene expression inside living cells
It allows the observation of biological processes in real time, as they happen.
This technique can be used as a noninvasive way to study protein trafficking, protein function, genetic regulatory or image bacteria, tumors and genes over a long period time.

9. Applications of bioluminescent imaging
Applications of bioluminescent imaging. (a) assessing the levels of trans-gene expression, (b) the location and extent of bacterial infection, (c) the efficiency of gene transfer and expression, and (d) the trafficking patterns of lymphocytes.