1. Evidence of Cholesterol Accumulated in High Curvature Regions: Implication to the Curvature Elastic Energy for Lipid Mixtures 
Wangchen Wang, Lin Yang, Huey W. Huang 
Biophysical Journal 
Volume 92, Issue 8, Pages (April 2007)
DOI: /biophysj
Copyright © 2007 The Biophysical Society Terms and Conditions

2. Figure 1
(Top) The detector image of x-ray reflection from a 2:1 mixture of di18:0(9,10Br)PC and cholesterol in the HIIδ phase. The inset shows a schematic of the diffraction geometry. (Bottom) The diffraction pattern was translated to the reciprocal space according to the diffraction geometry. The two reciprocal vectors are b1=(0.1314Å−1 sin 48.2°, Å−1 cos 48.2°) and b2=(0, Å−1). The solid green dots are the lattice defined by b1 and b2. The open circles are the mirror image of the lattice reflected by the mirror plane along the qz and perpendicular to qx—this left-right symmetry is due to the sample being a two-dimensional powder on the plane of the substrate. Due to the equality ǀb1ǀ=ǀb2ǀ, there are two mirror planes (purple lines) in the crystal lattice, one bisecting b1 and b2, another bisecting −b1 and b2.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2007 The Biophysical Society Terms and Conditions

3. Figure 2
MAD analyses for the detected peaks. For each independent peak, the square-root of the integrated intensity ǀFλǀ is plotted as a function of ǀf′λǀ/fn. The data are fit with a straight line, from which ǀF0ǀ2, ǀF2ǀ2, and the ratio F0/F2 are obtained. The results are in Table 1.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2007 The Biophysical Society Terms and Conditions

4. Figure 3
(Top) The Patterson function of Br amplitude ǀF2ǀ. The black line is the boundary of a unit cell. The dashed and solid lines indicate the direction of the major and minor axis, respectively. (Bottom) The Patterson profile along the minor axis (solid line) and along the major axis (dashed line) of a unit cell.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2007 The Biophysical Society Terms and Conditions

5. Figure 4
Comparison of the magnitudes of the diffraction amplitudes between the model and data. They are mutually normalized by the condition ∑i(Fimod)2=∑i(Fiexp)2.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2007 The Biophysical Society Terms and Conditions

6. Figure 5
The electron density distributions of Br and the whole lipid mixture shown in pairs, top and bottom, respectively. Each panel was constructed for one of 16 possible combinations of positive or negative phases for the four peaks (2,−1), (2,2), (−1,1), and (3,0). Panels 0 and 7 are based on the initial model phases and the final phases shown in Table 1, respectively. Out of all 16 combinations, only one, panel 7, satisfies the congruent condition between the bromine and the lipid distributions.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2007 The Biophysical Society Terms and Conditions

7. Figure 6
The details of the bromine (left) and the lipid mixture (right) distributions in one unit cell.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2007 The Biophysical Society Terms and Conditions

8. Figure 7
Schematic of the lipid distribution. The light blue region includes headgroups, glycerol backbones, and water molecules, bound by the interface. The chain region is divided into high Br regions (purple) and low Br regions (yellow). The volume ratio of the high Br region to the low Br region is 2:1. We found 80% of di18:0(9,10Br)PC and 13% of cholesterol in the high Br regions, and 20% of di18:0(9,10Br)PC and 87% of cholesterol in the low Br regions. The symbols of the majority molecule, PC in the purple region and cholesterol in the yellow regions, are depicted. Dotted red belts represent the high Br distribution. Point A is the peak position of PC headgroup density; the interface is defined to be 5Å away from A. Point B is the peak position of Br distribution. The measured distances are OA=4.5Å, OB=14.4Å, OC=19.5Å, OE=29.2Å, and CD=14.6Å.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2007 The Biophysical Society Terms and Conditions

9. Figure 8
(Left) Lamellar diffraction pattern of 2:1 mixture of di18:0(9,10Br)PC and cholesterol in the lamellar phase, measured by ω–2θ scan at 25°C and ∼90% RH. (Above ∼90% RH, the lamellar peaks began to broaden, similar to pure di18:0(9,10Br)PC as reported in (18).) Only one single lamellar series appears in the pattern. (Right) A two-dimensional ω-2θ scan around the second order indicates the peak has a single maximum. These data indicate the uniformity of the lamellar phase.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2007 The Biophysical Society Terms and Conditions