1. 研究介紹 生物醫學超音波影像實驗室
李百祺

2. 多元化學習！ 結合生物醫學與電子資訊科技已是前瞻研究趨勢！

3. 跨領域研究
很有趣
很有意義
很有挑戰性

4. What is Biomedical Engineering?
Interdisciplinary area of Biology, Medicine and Engineering.
Prevention, diagnosis and therapy of diseases.
Science, engineering and clinical applications.
Full of innovations and combination of the state of the arts.

5. 合作團隊： 臺大醫學院、生命科學院、基因體中心、奈米中心、國家衛生院、成大、師大、中正、暨南,…等單位。
Physics
Systems
Signal Processing
Ultrasonic Imaging:
Micro imaging
Breast imaging
Molecular imaging
Therapy:
Cavitation
Gene transfection
Photoacoustics:
Imaging
Nanoparticles
Systems:
Transducer
HW/SW
合作團隊： 臺大醫學院、生命科學院、基因體中心、奈米中心、國家衛生院、成大、師大、中正、暨南,…等單位。
跨領域研究：電子、物理、醫學、生命科學、化學、奈米材料。

6. Ultrasonic Micro-Imaging

7. Mouse Embryo Micro-Imaging
Depth (mm) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 P
Umbilical Cord
Width (mm)
-30
-20
-10 20 30
Velocity (mm/s)

8. Cover of IEEE Trans. on UFFC, Jan. 2004
Mouse Tumor Micro-Imaging
Cover of IEEE Trans. on UFFC, Jan. 2004

9. Ultrasound Assisted Therapy

10. Ｍicrobubbles and Cavitation
From UC Davis

11. Cavitation vs. Gene Transfection/Cell Viability

12. Photoacoustic Imaging and Gold Nanoparticles

13. Optoacoustic (Photoacoustic) Imaging
From Fairway
From TAMU

14. High frame rate photoacoustic imaging system
ultrasonic transducer
Lightguide
Cylindrical lens
Sample
This slice shows the schematic diagram of the high frame rate photoacoustic imaging system.
A Q-switched Nd:YAG laser operating at 1064 nm with a pulse duration of 8 ns was used for light illumination.
The laser beam was split and guided onto two multi-fiber light guides.
The output beams were then focused by two cylindrical lenses resulting in two 30 mm × 3 mm light lines.
Both the light guides and the focusing lenses were mounted on a fabricated holder as a part of the photoacoustic probe in order to confocally arrange the output light lines.
The generated photoacoustic signals were probed by a conventional array transducer, which was also confocally mounted between the two light guides on the photoacoustic probe.
The ultrasonic transducer has a pitch size of 0.3 mm and a center frequency of 5-MHz.
A reflecting foil with a thickness of 9 μm was stuck on the surface of the transducer in order to shield off the backscattered laser irradiation on the transducer surface.
Currently, the frame rate is limited by the pulse repetition rate of the laser system to 15 frames/s.
Laser system: Q-switched Nd:YAG
(wavelength 1064 nm, pulse duration 8 ns
Pulse repetition freq. 15 frames/s)
Lightguides: fiber bundle (15,000 fibers)
(output: 3 mm X 30 mm illumination area)
Photoacoustic probe:
Optical: Light guide with cylindrical lens
Acoustic: Linear array with 128 channels
(pitch : 0.3 mm, fc = 5 MHz)
Reflecting foil: 9 μm

15. Nanoprobe Designs A B 1000 nm 785 nm A.R.=3.7 A.R.=5.9 HER2Ab-AuNR785
CXCR4Ab-AuNR1000
A.R.=5.9 B
Our primary gold is to prepare two types of nanoprobes: AuNR785-HER2Ab and AuNR1000-CXCR4Ab, where the subscripts represent the absorption maxima, max’s, of two kinds of gold nanorods with different particle mean aspect ratios and Ab stands for antibody. The cell lines tested in our studies include (1) the MBT-WT cells (murine bladder cancer cell line-wild type) with overexpressed HER2 and (2) human hepatoma HepG2 cells with CXCR4 overexpressed on the cell surface. The transmembrane glycoprotein human epidermal growth factor receptor 2 (HER2; p185/neu, c-ErbB-2) was also found overexpressed in breast and ovarian cancer cells. CXC-chemokine receptor 4 (CXCR4) also functions as a coreceptors for human immunodeficiency virus (HIV) and is an attractive target for the development of anti-HIV drugs. Two nanoprobes suspended in aqueous solutions were carefully prepared to exhibit well separated absorption spectral bands for our multiple targeting experiment.

16. Molecular Imaging with Two Targets
Our primary gold is to prepare two types of nanoprobes: AuNR785-HER2Ab and AuNR1000-CXCR4Ab, where the subscripts represent the absorption maxima, max’s, of two kinds of gold nanorods with different particle mean aspect ratios and Ab stands for antibody. The cell lines tested in our studies include (1) the MBT-WT cells (murine bladder cancer cell line-wild type) with overexpressed HER2 and (2) human hepatoma HepG2 cells with CXCR4 overexpressed on the cell surface. The transmembrane glycoprotein human epidermal growth factor receptor 2 (HER2; p185/neu, c-ErbB-2) was also found overexpressed in breast and ovarian cancer cells. CXC-chemokine receptor 4 (CXCR4) also functions as a coreceptors for human immunodeficiency virus (HIV) and is an attractive target for the development of anti-HIV drugs. Two nanoprobes suspended in aqueous solutions were carefully prepared to exhibit well separated absorption spectral bands for our multiple targeting experiment.

17. Intravascular PA Imaging
Transit ring transducer
Catheter
Single element
IVUS transducer
Central frequency：11.43MHz
BW：15.75% at -6dB

18. Systems and Probes

19. System Development

20. High Frequency Transducer

21. 電機系的同學，在跨領域的環境中，發揮自己的專長
跨領域研究
很有趣
很有意義
很有挑戰性
電機系的同學，在跨領域的環境中，發揮自己的專長
nVIDIA for Medical Imaging
無線神經電刺激器
血管內的影像世界 ,…

22. 聯絡方式 博理館425室 02-33663551 paichi@ntu.edu.tw
生物醫學超音波影像實驗室: 明達館731室、博理館529室、慶齡中心、展書樓、奈米中心、基因體中心6F