Presentation is loading. Please wait.

Presentation is loading. Please wait.

Allergy multivaccines created by DNA shuffling of tree pollen allergens  Michael Wallner, PhD, Angelika Stöcklinger, MSc, Theresa Thalhamer, MSc, Barbara.

Published byÉva Magyar Modified over 5 years ago

Similar presentations

Presentation on theme: "Allergy multivaccines created by DNA shuffling of tree pollen allergens  Michael Wallner, PhD, Angelika Stöcklinger, MSc, Theresa Thalhamer, MSc, Barbara."— Presentation transcript:

1 Allergy multivaccines created by DNA shuffling of tree pollen allergens 
Michael Wallner, PhD, Angelika Stöcklinger, MSc, Theresa Thalhamer, MSc, Barbara Bohle, PhD, Lothar Vogel, PhD, Peter Briza, PhD, Heimo Breiteneder, PhD, Stefan Vieths, PhD, Arnulf Hartl, PhD, Adriano Mari, MD, Christof Ebner, MD, Peter Lackner, PhD, Peter Hammerl, PhD, Josef Thalhamer, PhD, Fatima Ferreira, PhD  Journal of Allergy and Clinical Immunology  Volume 120, Issue 2, Pages (August 2007) DOI: /j.jaci Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Deduced amino acid sequence alignment of chimeric proteins TPC7 and TPC9 with 3 of 14 parental allergens used for DNA shuffling. Alignment was performed with ClustalW/EMBL-EBI. Dashes indicate identical amino acids. The immunodominant T-cell epitope of Bet v 1 is highlighted. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 IgE binding of TPCs. A, ELISA with sera from patients with birch pollen allergy. Data are presented as mean OD405/492 units ± SD. P values were calculated by using paired-samples t tests (∗∗P < .01). B, Mediator release assay with RBL-2H3 cells. Protein concentrations (in nanograms per milliliter) to obtain 50% of maximum degranulation were determined. P values were calculated by using the Wilcoxon rank sum test (∗P < .05). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Immunogenicity of selected TPCs. A, Proliferative responses of PBMCs stimulated with parental allergens or chimeric proteins. Symbols represent individual patients, and bars represent medians. P values were calculated by using paired-samples t tests (∗P < .05). B, Proliferative responses of Bet v 1–specific TCLs A, B, and C to rBet v 1a, TPC7, or TPC9. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Genetic immunization. A, IgG responses after immunization with chimeric genes (up arrows) and sensitization with rBet v 1a (down arrows). B, Mediator release in RBL cells. P values were calculated with t tests (∗P < .05; ∗∗P < .01). C, ELISpot analysis. Data are expressed as mean cytokine-secreting cells per 106 spleen cells ± SEM. P values were calculated with t tests (∗P < .05). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Allergy multivaccines created by DNA shuffling of tree pollen allergens  Michael Wallner, PhD, Angelika Stöcklinger, MSc, Theresa Thalhamer, MSc, Barbara."

Similar presentations

Biologic allergen assay for in vivo test allergens with an in vitro model of the murine type I reaction  Andreas Hoffmann, VMD, Stefan Vieths, PhD, Dieter.

Biologic allergen assay for in vivo test allergens with an in vitro model of the murine type I reaction  Andreas Hoffmann, VMD, Stefan Vieths, PhD, Dieter.
Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.
Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies  Marija Geroldinger-Simic, MD, Thomas Zelniker,

Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies  Marija Geroldinger-Simic, MD, Thomas Zelniker,
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R
High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,

High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,
Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,

Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,
Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.

Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.
Martin Wolf, PhD, Lorenz Aglas, PhD, Teresa E

Martin Wolf, PhD, Lorenz Aglas, PhD, Teresa E
Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.

Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.
Sarcoplasmic calcium-binding protein is an EF-hand–type protein identified as a new shrimp allergen  Rosalía Ayuso, MD, PhD, Galina Grishina, MS, María.

Sarcoplasmic calcium-binding protein is an EF-hand–type protein identified as a new shrimp allergen  Rosalía Ayuso, MD, PhD, Galina Grishina, MS, María.
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
IgE cross-reactivity between the major peanut allergen Ara h 2 and the nonhomologous allergens Ara h 1 and Ara h 3  Merima Bublin, PhD, Maria Kostadinova,

IgE cross-reactivity between the major peanut allergen Ara h 2 and the nonhomologous allergens Ara h 1 and Ara h 3  Merima Bublin, PhD, Maria Kostadinova,
Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy  Margarete Focke-Tejkl, PhD, Milena.

Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy  Margarete Focke-Tejkl, PhD, Milena.
Plantago lanceolata: An important trigger of summer pollinosis with limited IgE cross- reactivity  Gabriele Gadermaier, PhD, Stephanie Eichhorn, MSc, Eva.

Plantago lanceolata: An important trigger of summer pollinosis with limited IgE cross- reactivity  Gabriele Gadermaier, PhD, Stephanie Eichhorn, MSc, Eva.
Delineating the specificity of an IgE-encoding transcriptome

Delineating the specificity of an IgE-encoding transcriptome
Elisabeth Roesler, MSc, Richard Weiss, PhD, Esther E

Elisabeth Roesler, MSc, Richard Weiss, PhD, Esther E
Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy  Julie Wang, MD,

Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy  Julie Wang, MD,
Birch pollen immunotherapy results in long-term loss of Bet v 1–specific TH2 responses, transient TR1 activation, and synthesis of IgE-blocking antibodies 

Birch pollen immunotherapy results in long-term loss of Bet v 1–specific TH2 responses, transient TR1 activation, and synthesis of IgE-blocking antibodies 
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?

Similar presentations

Ads by Google