1. Induction of OVA-specific CTLs by DCs
Induction of OVA-specific CTLs by DCs. DCs were incubated with soluble OVA in the absence (circles) or presence of chloroquine (squares), PMA (triangles), or both chloroquine and PMA (diamonds).
Induction of OVA-specific CTLs by DCs. DCs were incubated with soluble OVA in the absence (circles) or presence of chloroquine (squares), PMA (triangles), or both chloroquine and PMA (diamonds). Alternatively, DCs were loaded with the SIINFEKL peptide in the absence (circles) or presence of chloroquine (squares) and injected into groups of four mice, as described in Materials and Methods. Nine days later, spleens were removed and stimulated in vitro with SIINFEKL-loaded, irradiated splenocytes. After 5 days, a 51Cr release assay using SIINFEKL-pulsed EL4 (closed symbols) and EL4 (open symbols) targets was performed. The results are expressed as the means ± standard deviations. *, P < 0.05, or **, P < 0.01, versus untreated mice.
Bruno Garulli et al. Clin. Vaccine Immunol. 2008; doi: /CVI