1. CD4+ cells are required to generate protective immunity against the MT-HA tumor in HA104 mice.
CD4+ cells are required to generate protective immunity against the MT-HA tumor in HA104 mice. Spleen and lymph node cells were harvested from untreated BALB/c mice and suspended in PBS. A, CD4-expressing cells were removed by treatment with anti-CD4 mAb followed by immunomagnetic sorting as described in “Materials and Methods.” The resulting CD4-depleted cells were delivered (3.3 × 107 cells) by retro-orbital injection into HA104 mice. HA104 mice receiving whole-pooled spleen and lymph node cells or no cell transfers served as controls. Later the same day, mice were immunized with 106 irradiated (5000 rad) MT-HA cells administered s.c. as described in the legend to Fig. 1 <$REFLINK> . Mice were challenged 14 days later with 106 viable MT-HA cells and followed for tumor progression. B, mice were adoptively transferred as described above using nondepleted spleen and lymph node cells from untreated BALB/c mice. These mice were subsequently treated with anti-CD4 mAb for 3 days beginning 12 days after immunization to deplete CD4 T cells as described in “Materials and Methods.” On the third day of antibody treatment, mice were challenged with 106 viable MT-HA cells as in A and B and followed for tumor progression. Number of mice examined for each data point is shown in parentheses.
Steven C. Eck, and Laurence A. Turka Cancer Res 2001;61:
©2001 by American Association for Cancer Research