Presentation is loading. Please wait.

Presentation is loading. Please wait.

Hydroxychloroquine preferentially induces apoptosis of CD45RO+ effector T cells by inhibiting autophagy: A possible mechanism for therapeutic modulation.

Published byRoger Armand Vachon Modified over 4 years ago

Similar presentations

Presentation on theme: "Hydroxychloroquine preferentially induces apoptosis of CD45RO+ effector T cells by inhibiting autophagy: A possible mechanism for therapeutic modulation."— Presentation transcript:

1 Hydroxychloroquine preferentially induces apoptosis of CD45RO+ effector T cells by inhibiting autophagy: A possible mechanism for therapeutic modulation of T cells  Jorg van Loosdregt, PhD, Roberto Spreafico, PhD, Maura Rossetti, PhD, Berent J. Prakken, MD, PhD, Martin Lotz, MD, PhD, Salvatore Albani, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 131, Issue 5, Pages e1 (May 2013) DOI: /j.jaci Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 HCQ impairs autophagy in CD4+ T cells. A, Autophagy in human CD4+ cells, cultured in the presence of HCQ, n = 8. B, Histograms of Fig 1, A. C, Autophagosome visualization in CD4+ cells, cultured with 20 μmol/L of HCQ. D, Quantification of Fig 1, C, n = 3. E, p62, LC3, and ß-actin expression in CD4+ cells, n = 4. F, Autophagy in human CD4+CD45RA+CD45RO− and CD4+CD45RA−CD45RO+ cells, cultured with HCQ, n = 7. MFI, Mean fluorescent intensity. *P < .05. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 HCQ treatment preferentially induces apoptosis in CD4+CD45RA−CD45RO+ T cells. A, Apoptosis (by Annexin V staining) in human CD4+ cells, cultured in the presence of HCQ, n = 8. B, Histograms of Fig 2, A. C, Apoptosis in human CD4+CD45RA+CD45RO− and CD4+CD45RA−CD45RO+ cells, n = 7. D, FACS plot of HCQ-treated CD4+CD45RA+CD45RO− (blue) and CD4+CD45RA−CD45RO+ (red) cells stained with propidium iodide (PI). E and F, Sorted CD4+CD45RA+ (CFSE) or CD4+CD45RO+ (EF670) cells were mixed at a 1:1 ratio and cultured in the presence of HCQ (n = 4). CFSE, Carboxyfluorescein succinimidyl ester; FACS, fluorescence-activated cell sorting. ∗P < .05. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Hydroxychloroquine preferentially induces apoptosis of CD45RO+ effector T cells by inhibiting autophagy: A possible mechanism for therapeutic modulation."

Similar presentations

Joint modeling of parentally reported and physician-confirmed wheeze identifies children with persistent troublesome wheezing Danielle C.M. Belgrave, MSc,

Joint modeling of parentally reported and physician-confirmed wheeze identifies children with persistent troublesome wheezing Danielle C.M. Belgrave, MSc,
Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,
Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.

Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.
Endoplasmic reticulum stress influences bronchial asthma pathogenesis by modulating nuclear factor κB activation So Ri Kim, MD, PhD, Dong Im Kim, MS, Mi.

Endoplasmic reticulum stress influences bronchial asthma pathogenesis by modulating nuclear factor κB activation So Ri Kim, MD, PhD, Dong Im Kim, MS, Mi.
Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD 

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD 
Alveolar nitric oxide and asthma control in mild untreated asthma

Alveolar nitric oxide and asthma control in mild untreated asthma
Increased phenotypic differentiation and reduced corticosteroid sensitivity of fibrocytes in severe asthma  Chun-Yu Lo, MD, Charalambos Michaeloudes,

Increased phenotypic differentiation and reduced corticosteroid sensitivity of fibrocytes in severe asthma  Chun-Yu Lo, MD, Charalambos Michaeloudes,
Fatty acids, inflammation, and asthma

Fatty acids, inflammation, and asthma
Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.
Advances in food allergy in 2015

Advances in food allergy in 2015
Rigid substrate induces esophageal smooth muscle hypertrophy and eosinophilic esophagitis fibrotic gene expression  Eugene Tkachenko, PhD, Renee Rawson,

Rigid substrate induces esophageal smooth muscle hypertrophy and eosinophilic esophagitis fibrotic gene expression  Eugene Tkachenko, PhD, Renee Rawson,
A novel mutation in CD132 causes X-CID with defective T-cell activation and impaired humoral reactivity  Taco W. Kuijpers, MD, PhD, Paul A. Baars, PhD,

A novel mutation in CD132 causes X-CID with defective T-cell activation and impaired humoral reactivity  Taco W. Kuijpers, MD, PhD, Paul A. Baars, PhD,
Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 
Prostaglandin E2 suppresses CCL27 production through EP2 and EP3 receptors in human keratinocytes  Naoko Kanda, MD, PhD, Hiroshi Mitsui, MD, PhD, Shinichi.

Prostaglandin E2 suppresses CCL27 production through EP2 and EP3 receptors in human keratinocytes  Naoko Kanda, MD, PhD, Hiroshi Mitsui, MD, PhD, Shinichi.
Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.
Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells  Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.

Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells  Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
Desensitization to Chemotherapeutic Agents

Desensitization to Chemotherapeutic Agents
Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD 

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD 
Lung dendritic cells induce TH17 cells that produce TH2 cytokines, express GATA-3, and promote airway inflammation  Marianne Raymond, PhD, Vu Quang Van,

Lung dendritic cells induce TH17 cells that produce TH2 cytokines, express GATA-3, and promote airway inflammation  Marianne Raymond, PhD, Vu Quang Van,

Similar presentations

Ads by Google