1. Flow cytometric analysis of ROS production in oligonucleotide-treated PC3 prostate cancer cells as demonstrated by the oxidation of H2DCF → DCF and HE → E. Cells were treated with oligomer (400 nm)/Lipofectin (15 μg/ml) complexes for 3 days as described. a ...
Flow cytometric analysis of ROS production in oligonucleotide-treated PC3 prostate cancer cells as demonstrated by the oxidation of H2DCF → DCF and HE → E. Cells were treated with oligomer (400 nm)/Lipofectin (15 μg/ml) complexes for 3 days as described. a and b, representative histograms of the mean fluorescence channels of DCF and E fluorescence from one of three experiments. G3139 and 2006 caused a significant shift (more than 2-fold increase) in fluorescence of both markers of ROS production. c, a summary of ROS production by different oligomers. Fold increase in mean fluorescence channel was normalized against untreated cells. Columns, average; bars, SD (n = 3). * and Δ, P < 0.05, by Student's t test assuming unequal variances. (Control not included in analysis: All comparisons made to G4126 to eliminate nonspecific oligonucleotide treatment.)‏
Jonathan C. Lai et al. Mol Cancer Ther 2003;2:
©2003 by American Association for Cancer Research