1. Inhibitory effect of SFN pretreatment on the TPA-induced transformation of shMock- and shNrf2-transfected JB6 P+ cells.
Inhibitory effect of SFN pretreatment on the TPA-induced transformation of shMock- and shNrf2-transfected JB6 P+ cells. Cells (1 × 105/10-cm dish) were treated with SFN (0–5.0 μmol/L) for 5 days. The pretreated cells (at a density of 8,000 cells/well) were then transferred to soft agar containing TPA in 6-well plates for an additional 14 days. The colonies exhibiting anchorage-independent growth were counted under a microscope using ImageJ software. The graphical data are presented as the average of triplicate results from 2 independent experiments. #, P < 0.01 indicating a significant decrease or increase in colony formation compared with the JB6-shMock cells treated with TPA alone in soft agar.
Zheng-Yuan Su et al. Cancer Prev Res 2014;7:
©2014 by American Association for Cancer Research