1. Cotransport of GRAB with ca Rab11A in an axon.
Cotransport of GRAB with ca Rab11A in an axon. A, mStr-GRAB was cotransfected with EGFP-ca Rab11A into cortical neurons at 2 DIV, and the transport of GRAB and ca Rab11A was observed in the axons at 4 DIV. ca Rab11A was visualized as EGFP fluorescence (middle panels) and GRAB as mStr fluorescence (left panels). Arrows, arrowheads, and asterisks indicate the three vesicles labeled by GRAB and Rab11A. Scale bar, 5 μm. Bottom panels, Kymographs for a 32 s observation period. The cell body is to the left. B, Percentage of moving or stationary Rab11A-positive vesicles in the axon. Data are mean ± SEM. ca Rab11A-labeled vesicles, n = 205; GRAB/ca Rab11-labeled vesicles, n = 200. **p < C, Percentage of Rab11A-positive endosomes moving anterogradely, retrogradely, or bidirectionally. ca Rab11A-labeled vesicles, n = 156; GRAB/ca Rab11-labeled vesicles, n = 171. *p < D, Velocity of Rab11A-positive endosomes in the axon. ca Rab11A-labeled vesicles, n = 75; GRAB/ca Rab11-labeled vesicles, n = 88.
Kotaro Furusawa et al. J. Neurosci. 2017;37:
©2017 by Society for Neuroscience