1. CD4+ and CD8+ TILs express surface CD137.
 CD4+ and CD8+ TILs express surface CD137. BALB/c mice bearing CT26-derived subcutaneous tumors for 12 to 14 days were euthanized to prepare single-cell suspensions from tumor nodules, spleens, and lymph nodes. A, FACS analysis of CD137 expression on gated CD4 or CD8 T cells from spleen, lymph node, or tumor tissue as indicated. Tinted histograms represent isotype-matched control antibodies and open histograms CD137-specific surface staining from a representative case acquired with an analogic FACSCalibur. As a control to rule out unspecific immunostaining on CD3+ TILs, TILs from CT26-derived tumors grafted onto CD137-/- mice were not stained by the anti-CD137 mAbs used for detection. B, summary of data of 5 mice. LN, lymph node. C, double immunofluorescence for CD137 and either CD4 or CD8 TILs on CT26 tumor tissue sections. Data are representative of at least 15 microscopic fields in 3 different sections from 3 independent tumors.
Asís Palazón et al. Cancer Discovery 2012;2:
©2012 by American Association for Cancer Research