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Distribution Patterns of Postmortem Damage in Human Mitochondrial DNA

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Presentation on theme: "Distribution Patterns of Postmortem Damage in Human Mitochondrial DNA"— Presentation transcript:

1 Distribution Patterns of Postmortem Damage in Human Mitochondrial DNA
M. Thomas P. Gilbert, Eske Willerslev, Anders J. Hansen, Ian Barnes, Lars Rudbeck, Niels Lynnerup, Alan Cooper  The American Journal of Human Genetics  Volume 72, Issue 1, Pages (January 2003) DOI: /345378 Copyright © 2003 The American Society of Human Genetics Terms and Conditions

2 Figure 1 Powder-extraction method. a, Encasement of tooth, crown down, in liquid silicone, which is left to set. b, Exposure of root tip (5–10 mm) by using horizontal cuts into the silicone. c, Horizontal removal of root tip, flush against silicone surface, with sterile cutting disk. d, Extraction of sterile pulp-cavity powder by drilling into root from cut surface. The American Journal of Human Genetics  , 32-47DOI: ( /345378) Copyright © 2003 The American Society of Human Genetics Terms and Conditions

3 Figure 2 Human mitochondrial regions amplified
The American Journal of Human Genetics  , 32-47DOI: ( /345378) Copyright © 2003 The American Society of Human Genetics Terms and Conditions

4 Figure 3 Damage variation across HVR1 and COIII. a, Absolute damage measured in hits per site for data sets generated by each PCR primer set across HVR1 (16055–16410). b, Relative-rate variation (×100) across HVR1, calculated as in text. Four areas of interest are marked onto the graph. c, Absolute damage across region of COIII (09500–09632). Numbering is with reference to the CRS (Anderson et al. 1981). The American Journal of Human Genetics  , 32-47DOI: ( /345378) Copyright © 2003 The American Society of Human Genetics Terms and Conditions

5 Figure 4 Observed and expected frequency of absolute damage rates for HVR1 MR (a), HVR1 OR (b), and COIII (c) sites. For full details, see text. The American Journal of Human Genetics  , 32-47DOI: ( /345378) Copyright © 2003 The American Society of Human Genetics Terms and Conditions

6 Figure 5 Modification of mitochondrial HVR1 haplogroup tree (reprinted, with permission, from Richards and Macaulay 2000) to demonstrate potential for haplogroup misidentification. From a starting sample of haplogroups H or V, arrows indicate the direction along the tree a sample can move because of hydrolytic deamination (or real singleton substitution) of C→T or A→G. Site numbers are with reference to the CRS (Anderson et al. 1981). Relative rates are calculated for the MR (underlined) and OR. For full details, see text. The American Journal of Human Genetics  , 32-47DOI: ( /345378) Copyright © 2003 The American Society of Human Genetics Terms and Conditions


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