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Ki-67 expression is dynamically controlled by a cell-cycle–regulatory network.
Ki-67 expression is dynamically controlled by a cell-cycle–regulatory network. A, Scheme. HDFs were sequentially synchronized over two cell cycles upon entry from quiescence. B, DNA content was analyzed by flow cytometry. C, qRT-PCR analysis of indicated mRNA. D, Western blotting of indicated proteins (Q, quiescence; S, serum stimulation; THY, thymidine block; THY +, time point after release from thymidine block; RO3306, RO3306 block; RO +, time point after release from RO3306 block). LC, loading control (amido black). Michal Sobecki et al. Cancer Res 2017;77: ©2017 by American Association for Cancer Research
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