1. A His/Pro-rich fragment of HRG is present in human tissue.
A His/Pro-rich fragment of HRG is present in human tissue. A. Schematic illustration of HRG. Binding epitopes of antibodies 0116, 0119, and 0115, respectively, are indicated by a line. B. Immunohistochemical staining of a human kidney tumor using the FITC-conjugated lectin UEA-1 to detect blood vessels (green) together with antibodies 0116 (left), 0119 (middle), and 0115 (right). C. Confocal microscope analysis of paraffin-embedded human kidney tumor tissue, coimmunostained with 0119 (red) and the vascular marker CD31 (green). Blue, nuclei. D. Immunohistochemical staining of a human kidney tumor after preincubation of the antibody 0119 with the peptide it was raised against. E. HRG purified from human plasma (5 or 20 μg) was immunoprecipitated (IP) using equal amounts of the antibodies 0115, 0116, and 0119 and immunoblotted (IB) with a mouse monoclonal antibody against HRG (MO37, Takara). Scale bars, 20 μm.
Åsa Thulin et al. Mol Cancer Res 2009;7:
©2009 by American Association for Cancer Research