1. Biochemistry lab revision
Nada Mohamed Ahmed , MT (ASCP)i

2. principles of the experiments
Substances
Indicator (reagent)
Name of experiments
Carbohydrates
(Monosaccharides)
Benedicts reagent
Benedicts test
Starch (Polysaccharides)
iodine/potassium iodide
Iodine Test
lipids
Sudan IV
ethanol (an organic solvent)
Emulsion Test For Lipids
protein
biuret reagent
Biuret test
tyrosine amino acid
Folin's reagent
Folin's test
plasma protein
benzidine
Benzidine test
hemoglobin
Drabkin’s reagent
Spectrophotometric

3. principles of the experiments
Test
Principle
Benedicts test
The reagent contains blue copper (II) ions.
In the presence of a reducing sugar these are reduced to red copper (I) ions. These form a precipitate which is insoluble in water.
Sudan IV
Sudan IV is not soluble in water; it is, however, soluble in lipids.
Sudan IV is added to a solution to dissolve any possible lipids.
Biuret test
Under alkaline conditions substances containing two or more peptide bonds form a purple complex with copper salts in the reagent.

4. Define : 
Reducing sugar: .……………………………………………………………………………………………………

5. Q-2- complete the table Name of the reagent Substance Carbohydrate
Emulsion test
Tyrosin

6. Q-3- True or False 1-sucrose is a reducing sugar[ ]
1-sucrose is a reducing sugar[ ]
2-lipides are soluble in organic solvents[ ]
3- protein are optically inactive [ ]