1. A, high-resolution methylation analysis of ZNF545 promoter by BGS in representative carcinoma cell lines.
A, high-resolution methylation analysis of ZNF545 promoter by BGS in representative carcinoma cell lines. B, restored expression of ZNF545 in tumor cell lines treated with Aza or combined with TSA, accompanied with demethylation of the promoter. M, methylated; U, unmethylated. C, ZNF545 expression was restored in HCT116 cell lines with genetic double knockout of DNMT1 and DNMT3B (DKO), but not in single knockout of DNMT1 (1KO) or DNMT3B (3KO). Demethylation of ZNF545 promoter was also observed in HCT116-DKO cells. D, detailed BGS analysis further confirmed the demethylation of ZNF545 promoter in HCT116-DKO cells. The ZNF545 TSS is shown as bent arrows. Circles, CpG sites analyzed; row of circles, an individual promoter allele that was cloned, randomly selected and sequenced; filled circle, methylated CpG site; open circle, unmethylated CpG site.
Yingduan Cheng et al. Mol Cancer Res 2012;10:
©2012 by American Association for Cancer Research