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Increased Type 1 Immune Response in the Bone Marrow Immune Microenvironment of Patients with Poor Graft Function after Allogeneic Hematopoietic Stem Cell.

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Presentation on theme: "Increased Type 1 Immune Response in the Bone Marrow Immune Microenvironment of Patients with Poor Graft Function after Allogeneic Hematopoietic Stem Cell."— Presentation transcript:

1 Increased Type 1 Immune Response in the Bone Marrow Immune Microenvironment of Patients with Poor Graft Function after Allogeneic Hematopoietic Stem Cell Transplantation  Yu-Tong Wang, Yuan Kong, Yang Song, Wei Han, Yuan-Yuan Zhang, Xiao-Hui Zhang, Ying-Jun Chang, Zheng-Fan Jiang, Xiao-Jun Huang  Biology of Blood and Marrow Transplantation  Volume 22, Issue 8, Pages (August 2016) DOI: /j.bbmt Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions

2 Figure 1 Immunophenotypic analysis of Tc1, Tc2, Th1, and Th2 cells. The lymphocytic population was initially defined using a forward (FSC) and side scatter (SSC) gate (A). CD4+ T cells and CD8+ T cells were then gated based on the CD8 antigen (B). Representative fluorescence-activated cell sorting analysis of CD8−IFN-γ+ Th1 cells and CD8+IFN-γ+ Tc1 cells from a PGF patient (C), a GGF patient (D), an HD, and (E) CD8−IL-4+ Th2 cells and CD8+L-4+ Tc2 cells from a PGF patient (F), a GGF patient (G), and an HD (H) are provided. Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt ) Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions

3 Figure 2 IFN-γ (A) and IL-4 (B) levels in BM plasma, as measured by cytometric beads assay. The PGF patients exhibited significantly elevated plasma IFN-γ levels compared with HD and decreased plasma IL-4 levels compared with GGF patients and HD. Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt ) Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions

4 Figure 3 The percentages of Th1 (A) and Th2 (B) cells among CD4+ T cells and Tc1 (D) and Tc2 (E) cells among CD8+ T cells. Th1/Th2 (C) and Tc1/Tc2 (F) ratios are indicated. Statistical analyses were performed using the Mann-Whitney U test. Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt ) Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions


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