1. Antibody staining of SuUR protein.
Antibody staining of SuUR protein. (A) The antibody recognizes an additional band (*) in the H7 stock (hs-SuUR) after heat shock. Adult H7 flies were heat-shocked for 45 min at 37° and allowed to recover for 1 or 2 hr before extraction. The endogenous SuUR band is extremely weak in the w67 extracts and a difference with the SuUR mutant is barely perceptible. The band of 110 kD visible in all extracts is due to nonspecific staining because it is not recognized by antibodies from a second rabbit. The sizes of molecular weight markers (middle lane) are shown on the left. (B and C) The anti-SuUR antibody stains the chromocenter (CH) and ~110 euchromatic sites on salivary gland polytene chromosomes of the y, w67 stock (shown in reversed contrast). The stained regions identified correspond well with late-replicating sites. (D) Overproduction of SuUR protein after heat shock of H7 larvae results in appearance of additional strong signals (shown in reversed contrast). The chromocenter (CH) stains strongly.
I. V. Makunin et al. Genetics 2002;160:
Copyright © 2002 by the Genetics Society of America