1. Paper review
Miyeon Shin
21 Apr 2015

2. Reason for selection

3. Materials and Methods Rainbow trout n = 15 (Total n = 60)
1) Design of In Vivo study
Conc. of spiked food
:BDE 209
[940±14 ng/g w.w]
Dosing volume
: 1% of B.W/day
Dosing duration
: 5 months
(5 days per week)
Sample type
: Blood, liver, intesti
-nal tissue, carcass
Exposure Gr.
Control Gr.

4. Materials and Methods Used cell line : 1) Liver of rainbow trout
1) Design of In Vitro study
Common carp
Used cell line : 1) Liver of rainbow trout
2) common carp

5. Results and Discussion 1) In Vivo experiment

6. Results and Discussion 1) In Vivo experiment
Steady state
3.5 month

7. Results and Discussion 1) In Vivo experiment
Lipid normalized BDE 209 *
* Lipid content was assumed.

8. Results and Discussion 1) In Vivo experiment$
* NonaBDEs
$ OctaBDEs
& HeptaBDEs $ *
ECNI (electron captgure negative ion)
& $ $
&
& $ * *
& $

9. Results and Discussion 1) In Vivo experiment
ECNI (electron captgure negative ion)

10. Results and Discussion 1) In Vivo experiment
Kierkegaard et al., (1999) ES&T
Input dose
9.4 μg of BDE 209/kg body weight/day
무지개송어의 근육에서 BDE209의 debromination을 관찰, 시간에 따라 증가하는 것이 보임.

11. Conceptual model of potential metabolic transformation pathways for decaBDE in fish

12. Results and Discussion 1) In Vivo experiment
Consist of
Data on day 112
202 (19%),
201 (9%)
32%
26%
208
(31%)
94%
37%
68%
Approx.
30%

13. Results and Discussion 1) In Vivo experiment
Due to the size or higher serum protein binding affinities of BDE 209
209
208
207
206 .
Diffusion
Liver Tissue for Fish
Place for biotransform. of chemicals
Preferential accumul.
of lighter congeners
BDE 209 -> lower congeners

14. Results and Discussion 1) In Vivo experiment
Log Kow increases with the degree of bromination
Log Kow: BDE 209 (10)
octaBDE ( )
pentaBDE ( )
tetraBDE ( )
Easy to disposition in liver or serum
Ref) Eljarrat et al., Brominated Flame Retardants- the handbook of env. chem.

15. Results and Discussion 1) In Vivo experiment
Common carp Rainbow trout
The previous study (2004)
The present study (2006)

16. Results and Discussion 1) In Vivo experiment
Both fish have the capability
to debrom. BDE209,
But differ in the extent and
Probably the rate of debom.
Common carp
BDE 202
There’s no BDE209 in whole body of the carp -> Carp have an enhanced metabolic ability
Ref) Stapleton et al., (2004), ES&T 38:
In vitro test

17. Ref) 1) Wikipedia, 2)

18. Results and Discussion 1) In Vitro experiment
Incubated for
1hr and 24hr
At 25℃
BDE 209
15 pmole/mg
of protein
Control 실험내용 및 결과에 대해서도 언급할 것

19. Results and Discussion 1) In Vitro experiment
Biotransformed
65% of BDE209
Biotransformed
22% of BDE209

20. Results and Discussion 1) In Vitro experiment
Rainbow trout Common carp
In vitro
In vivo

21. Deiodinase(DI) enzymes
DI contributes to breakdown of thyroid hormones.
It releases iodine.