Presentation is loading. Please wait.

Presentation is loading. Please wait.

Fig. 6 Zic5 regulates glucose metabolism in a β-catenin/Tcf7l2–dependent manner in HCT116 colon cancer cells. Zic5 regulates glucose metabolism in a β-catenin/Tcf7l2–dependent.

Published byLogan Boyd Modified over 4 years ago

Similar presentations

Presentation on theme: "Fig. 6 Zic5 regulates glucose metabolism in a β-catenin/Tcf7l2–dependent manner in HCT116 colon cancer cells. Zic5 regulates glucose metabolism in a β-catenin/Tcf7l2–dependent."— Presentation transcript:

1 Fig. 6 Zic5 regulates glucose metabolism in a β-catenin/Tcf7l2–dependent manner in HCT116 colon cancer cells. Zic5 regulates glucose metabolism in a β-catenin/Tcf7l2–dependent manner in HCT116 colon cancer cells. (A) Zic5 KO cells (C18) were infected with three independent shRNAs against Zic2. Western blot showed the knockdown efficiency of Zic2 knockdown after puromycin selection. HCT116 parental cells were included as positive control. (B) RNA-seq tracks showed the GLUT1/SLC2A1 gene expression decrease with Zic2 knockdown. (C) Western blot showing the cleaved PARP levels of Zic2 knockdown in Zic5 KO cells cultured in the DMEM medium with high (H, 25 mM) or low glucose (L, 0.5 mM). (D) Glucose uptake in Zic5 KO cells with shCtrl of shZic2 is measured by 2-NBDG uptake. The fluorescence intensity is quantified by the plate reader (n = 8). ***P < (E) Venn diagram analysis identifies 58 common genes by Zic2 knockdown and Zic5 KO. The P value was determined with the hypergeometric test. (F) Zic5 acts as a transcriptional repressor in regulating SLC2A1/GLUT1 gene expression. Loss of Zic5 derepresses GLUT1 gene expression and further increases the protein level of GLUT1. Glucose uptake is increased and thus leads to the more glycolytic features of the cells. This may link to the tumor progression in the tumor microenvironment. Zibo Zhao et al. Sci Adv 2019;5:eaax0698 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

Download ppt "Fig. 6 Zic5 regulates glucose metabolism in a β-catenin/Tcf7l2–dependent manner in HCT116 colon cancer cells. Zic5 regulates glucose metabolism in a β-catenin/Tcf7l2–dependent."

Similar presentations

Fig. 5 Correlation of RNA expression and protein abundance.

Fig. 5 Correlation of RNA expression and protein abundance.
Fig. 2 Transport properties of a BP transistor at low temperature.

Fig. 2 Transport properties of a BP transistor at low temperature.
Fig. 4 Ballistic simulation of BP FETs.

Fig. 4 Ballistic simulation of BP FETs.
Fig. 4 Restoration of MTAP expression produces heterogeneous effects on metabolism. Restoration of MTAP expression produces heterogeneous effects on metabolism.

Fig. 4 Restoration of MTAP expression produces heterogeneous effects on metabolism. Restoration of MTAP expression produces heterogeneous effects on metabolism.
Fig. 1 TET2 is a coactivator of ERα.

Fig. 1 TET2 is a coactivator of ERα.
Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,

Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,
Vibrational spectra of medieval human bones (Leopoli-Cencelle, Italy)

Vibrational spectra of medieval human bones (Leopoli-Cencelle, Italy)
Fig. 5 Thermal conductivity of n-type ZrCoBi-based half-Heuslers.

Fig. 5 Thermal conductivity of n-type ZrCoBi-based half-Heuslers.
Fig. 1 Map of water stress and shale plays.

Fig. 1 Map of water stress and shale plays.
Fig. 1 Examples of experimental stimuli and behavioral performance.

Fig. 1 Examples of experimental stimuli and behavioral performance.
Fig. 4 Resynthesized complex boronic acid derivatives based on different scaffolds on a millimole scale and corresponding yields. Resynthesized complex.

Fig. 4 Resynthesized complex boronic acid derivatives based on different scaffolds on a millimole scale and corresponding yields. Resynthesized complex.
HT synthesis of boronic acids using the building block approach

HT synthesis of boronic acids using the building block approach
Fig. 6 Comparison of properties of water models.

Fig. 6 Comparison of properties of water models.
Fig. 1 Mean and median RCR (Relative Citation Ratio) of Roadmap Epigenomics Program research articles for each year. Mean and median RCR (Relative Citation.

Fig. 1 Mean and median RCR (Relative Citation Ratio) of Roadmap Epigenomics Program research articles for each year. Mean and median RCR (Relative Citation.
Fig. 5 Conservation of m6A in mammals.

Fig. 5 Conservation of m6A in mammals.
Fig. 3 Glucose- and structure-dependent insulin release.

Fig. 3 Glucose- and structure-dependent insulin release.
m6A identification method based on m6A sensitive RNA endoribonuclease

m6A identification method based on m6A sensitive RNA endoribonuclease
Fig. 3 Rotation experiment, setup.

Fig. 3 Rotation experiment, setup.
Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.

Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.
Fig. 1 Schematic illustration of the preparation and potential application of FMSMs. Schematic illustration of the preparation and potential application.

Fig. 1 Schematic illustration of the preparation and potential application of FMSMs. Schematic illustration of the preparation and potential application.

Similar presentations

Ads by Google