1. Stress-induced release of inflammatory monocytes from the bone marrow into circulation was attenuated by MTP. Male C57BL/6 mice were injected daily with either vehicle or MTP (100 mg/kg) 30 min before control or RSD (Stress).
Stress-induced release of inflammatory monocytes from the bone marrow into circulation was attenuated by MTP. Male C57BL/6 mice were injected daily with either vehicle or MTP (100 mg/kg) 30 min before control or RSD (Stress). At 14 h after stress, brains were perfused, fixed, and labeled for ΔFosB. A, Representative images of ΔFosB expression in the prelimbic cortex (PrL). Scale bar, 125 μm. Inset, Region used for analysis. B, Average number of ΔFosB+ cells in the PrL (n = 3). C, Plasma corticosterone (n = 6). D, IL-6 levels (n = 3 or 4). E, Spleen weight (n = 6) was determined. Percentage of (F) CD11b+ Ly6Chi monocytes and (G) CD11b+ Ly6Cint granulocytes in the bone marrow. H, Percentage and (I) representative bivariate dot plots of CD11b+ and Ly6Chi labeling of monocytes in circulation. J, mRNA expression of CXCL12 in the bone marrow (n = 3 or 4). Error bars indicate mean ± SEM. Means with different letters (a, b, or c) are significantly different (p < 0.05) from each other.
Anzela Niraula et al. J. Neurosci. 2018;38:
©2018 by Society for Neuroscience