1. Laboratory Safety
and
Equipment

2. Before Lab Work, Get to Know:
Hazards of materials & agents and their prescribed safety procedures
Designated escape route and alternate
Location of safety equipment

3. How to Dress for Lab Pants (no ankles showing) Closed toed shoes
Hair tied up and away from face
Short sleeves
No synthetic clothing
No clothing with holes
No jewelry! Nothing in ears, necklaces or on the wrists/fingers

4. Internal Emergency Procedures
For emergencies requiring internal assistance, get your teachers attention fast by saying:
Teacher
code red
Report all Accidents (breakage), Injuries, Fires, Spills, and Close calls Immediately to your teacher!

5. Extinguishing a Fire Send someone to pull the fire alarm!
Then P.A.S.S.
Pull the pin
Aim the nozzle
Squeeze the handle
Sweep at the base of the fire

6. Eye Protection
Wear goggles to protect your eyes from splashes and fumes.
They fit over prescription eye wear.
They must cover your eyes, not your neck or hair!
Always wash your hands before leaving the lab.

7. Chemical Safety
Never pour chemicals back into their original containers.
They may be contaminated and could cause a reaction or contaminate your original substance.
Always alert your teacher of a spill!

8. Electrical Safety
Never handle electrical equipment with wet hands. Work areas, including floors and tables, should be dry.
Never overload an electrical circuit.
Make sure all electrical equipment is properly grounded.
Keep electrical cords away from areas where someone may trip on them, or where the cords can tip over laboratory equipment.

9. Tie back long hair and secure loose clothing when working around flames.  

10. Never point a chemical towards anyone.
Correct!
WRONG!

11. Raising your hand . . .
When you see me raise my hand I have something IMPORTANT to say!
You should:
Stop talking
Raise your hand
Look at the teacher
Make sure everyone near you is doing the same

12. Eating in the lab . . .
Don’t do it - Never taste anything during a laboratory activity. If an investigation involves tasting, it will be done in the cafeteria or in the room.  

13. Dress Appropriately . . .
If instructed to do so, wear your laboratory apron or smock to protect your clothing.
Wear closed toed shoes in the lab.

14. Cleaning Up . . . Clean your lab station when your done.
Put away all supplies as instructed.
Throw away all garbage.
Do Not put garbage in sinks!
Follow directions for
disposal of waste

15. Can you name the piece of equipment
Lab Equipment
What do you remember . . .
Can you name the piece of equipment
and its function?

16. Erlenmeyer Flask
It is used to contain reaction solutions.

17. Beaker
Beakers are the most versatile glassware in the lab and can be used for just about anything.
The volume graduations on beakers should be used only for "ballpark" estimates.

18. Glass Stirring Rod
A glass rod is used to manually stir solutions. It can also be used to transfer a single drop of a solution.

19. Beaker Tongs
These tongs are used for picking up beakers that may be too hot or too cold to touch.

20. Graduated cylinder
Used to make accurate measurements of liquid volumes.
The bumper ring on larger cylinders is to prevent breakage if tipped over. Keep it near the top.

21. Test Tube Test tubes hold small volume of liquid.
We will put these on test tube racks

22. Thermometer
A thermometer is used to measure temperatures.

23. Medicine Dropper
A medicine dropper is used to transfer a small volume of liquid (less than one mL).

24. Weighing Boat
Used to weigh solids that will be transferred to another vessel.

25. Microscope Used to magnify specimens.
You will use a compound light microscope that magnifies up to 1000x.

26. Inoculating Loop Used to transfer culture to a slide or Petri dish.
Sterilize in between uses.

27. Cover slip Microscope Slide Covers specimen on the slide.
Holds specimens for view under the microscope
Cover slip
Covers specimen on the slide.

28. Petri Dishes Can be used to temporarily hold specimens.
Its primary use is to culture bacteria (microorganisms) in agar.

29. Dissecting Kit Used for dissections
Contents: Scissors, probes, dropper, forceps, bone crushers, scalpel, and ruler
Contents may vary due to availability

30. Reading the Volume
Determine the volume of solution by reading the bottom of the meniscus at eye level. Record the volume using all certain digits and one uncertain digit. Certain digits are obtained from calibration marks. Uncertain digits (the last digit in the number) are estimated between calibration marks. Some examples are shown below:
                                             
4.88 mL reading
4.49 mL reading

31. Measure volume with a graduated cylinder
Examine the meniscus above and determine the volume of the liquid contained in the graduated cylinder. Answer

32. My Favorites Basic Science Lab Equipment Basic Science Lab Techniques
Lab safety movie
Lab Safety Rules
Molecular Expressions Software Museum of Microscopy Screen Saver - 80 Microscope Edition
SafetyEmporium.com Workplace Safety Awareness Signs
UW Lab Topics