1. In Vivo Gene Delivery to Synovium by Lentiviral Vectors
Elvire Gouze, Robert Pawliuk, Carmencita Pilapil, Jean-Noel Gouze, Christina Fleet, Glyn D. Palmer, Christopher H. Evans, Philippe Leboulch, Steven C. Ghivizzani 
Molecular Therapy 
Volume 5, Issue 4, Pages (April 2002)
DOI: /mthe
Copyright © 2002 American Society for Gene Therapy Terms and Conditions

2. FIG. 1
Schematic representation of the β-GEO(A) and IURA (B) lentiviral vectors. HIV LTR, human immunodeficiency virus long terminal repeat; ψ+, packaging signal; RRE, Rev-responsive element; cPPT/FLAP, central polypurine tract/DNA flap; PPT, polypurine tract. Expression of the gene of interest is under the control of the EF-1 α promoter.
Molecular Therapy 2002 5, DOI: ( /mthe )
Copyright © 2002 American Society for Gene Therapy Terms and Conditions

3. FIG. 2
Histochemical detection of β-galactosidase activity in synovium following the intra-articular injection in both knees of 5 × 107 iu β-GEO lentivirus (A), 5 × 107 pfu Ad.LacZ (B), or 5 × 107 iu of lentivirus containing no cDNA (C). Naive synovium is shown in (D). Wistar rats were killed 5 days postinjection, and the knees were dissected, stained for LacZ activity, and embedded in paraffin. Sections (7-μm) were stained with eosin and are shown at × 10 magnification.
Molecular Therapy 2002 5, DOI: ( /mthe )
Copyright © 2002 American Society for Gene Therapy Terms and Conditions

4. FIG. 3
Lentivirus-mediated delivery of IL1RA in vitro and in vivo. (A) In vitro expression of IL1RA following infection of 105 rat synovial cells using a range of multiplicities of infection (MOI) of IL1RA lentivirus. (B) In vivo expression of IL1RA after intra-articular injection of lentivirus into the knee joint of immunocompromised rats. IL1RA lentivirus was injected intra-articularly into the knee joints of athymic nude rats (filled bars) or normal Wistar rats (open bars). Rats were euthanized 5, 10, 20, and 42 days postinjection. Knees were dissected and incisions were made to allow exposure of the entire joint capsule. Joints were then placed in 24-well plates with 1 ml of DMEM and cultured for 24 hours. The levels of IL1RA released into the conditioned medium were measured by ELISA. Ex vivo culture of the knees of naive animals results in mean background levels of ± 14.3 pg/ml. Each bar represents mean values ± SD from eight knees of four rats (*P < 0.01 compared with IL1RA levels in Wistar rats, t-test).
Molecular Therapy 2002 5, DOI: ( /mthe )
Copyright © 2002 American Society for Gene Therapy Terms and Conditions

5. FIG. 4
Biodistribution of the IL1RA protein following the intra-articular injection of 5 × 107 iu IL1RA lentivirus. Rats were sacrificed 5 (gray bars) and 10 (black bars) days postinjection, and organs were harvested. A portion of tissue (approximately 100 mg) from each animal was minced with a scalpel and placed in 1 ml culture medium for 24 hours. Blood samples were collected by cardiac puncture and centrifuged, and plasma was stored at –20°C until testing. The levels of IL1RA released into the conditioned medium or present in the plasma were measured by ELISA and compared with levels from naive animals (clear bars). Each bar represents mean values ± SD from six rats (*P < 0.01, t-test).
Molecular Therapy 2002 5, DOI: ( /mthe )
Copyright © 2002 American Society for Gene Therapy Terms and Conditions