1. Method for anatomical characterization and quantification of arterial lumen and PVS. a, A three-dimensional image stack is obtained using two-photon microscopy through the thinned skull of a bAct-GFP mouse, with representative slices shown at the level of t...
Method for anatomical characterization and quantification of arterial lumen and PVS. a, A three-dimensional image stack is obtained using two-photon microscopy through the thinned skull of a bAct-GFP mouse, with representative slices shown at the level of the skull (blue is second harmonic generation created by bone), dura, pia, and brain (green). White line represents angle and position of the orthogonal reconstruction shown in b. b, Each cranial layer [including SAS (sas)], along with the arterial lumen (art) and arterial PVS (pvs). c, To quantify cross-sectional areas of PVS and lumen, the green GFP channel is thresholded and cross-sectional areas of PVS and lumen are measured from the result. PVS and lumen areas are normalized across time points to their average baseline size to compare across mice. d, Diagram of mouse skull showing the location of frontal craniotomy, where CSD was induced by pinprick (glass micropipette, black circle), and the thinned area in parietal bone (blue circle), where pial blood vessels and PVS were imaged.
Aaron J. Schain et al. J. Neurosci. 2017;37:
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