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Cytokines and growth factors inhibit tumor necrosis factor alpha–induced up-regulation of fibronectin binding on bovine endometrial cells Selvaraj Nataraja, Ph.D., David Kagan, M.S., Ann Clark, Ph.D., Stephen Palmer, Ph.D. Fertility and Sterility Volume 89, Issue 5, Pages (May 2008) DOI: /j.fertnstert Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions
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Figure 1 Effect of TNF-α on the adhesion of BEND cells to peritoneal cells. The BEND cells were incubated with TNF-α (5 ng/mL) either alone or in presence of TBP (10 μg/mL) for 72 hours. The BEND cells were fluorescently labeled and allowed to bind to mesothelial cells. Data are mean ± SD, n = 3. ∗P<.05. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions
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Figure 2 Dose-dependent effect of TNF-α on BEND cell binding to fibronectin conjugated FITC beads. (A) BEND cells were treated with increasing concentration of TNF-α for 24 hours, followed by the binding of fibronectin beads to BEND cells. (B) TBP dose-dependently inhibited TNF-α–induced the binding of fibronectin beads to BEND cells. Mean ± SD, n = 4. This experiment was repeated three times with similar results. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions
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Figure 3 Effect of IL-17 and IFN-α2 on TNF-α–induced binding of fibronectin to BEND cells. (A) Binding of fibronectin beads to BEND cells in the presence of 15 ng/mL of TNF-α. Dose-dependent inhibition by (B) IL-17, (C) IFN-α2a, and (D) IFN-α2b on TNF-α–induced binding of fibronectin to BEND cells. ∗P<.01 compared with control. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions
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Figure 4 Effect of TGF-β on TNF-α–induced binding of fibronectin to BEND cells. (A) Binding of fibronectin beads to BENDS cells in the presence of 15 ng/mL of TNF-α. (B) Dose-dependent effect of TGF-β on TNF-α–induced binding of fibronectin to BEND cells. ∗P<.01 compared with control. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions
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Figure 5 Effect of PDGF subtypes on TNF-α–induced binding of fibronectin to BEND cells. (A) Binding of fibronectin beads to BEND cells in the presence of 15 ng/mL of TNF-α. Dose-dependent effect of (B) PDGF-AA, (C) PDGF-BB, and (D) PDGF-AB on TNF-α–induced binding of fibronectin to BEND cells. ∗P<.01 compared with control. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions
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