1. BCX inhibits lamellipodia formation in lung cells.
BCX inhibits lamellipodia formation in lung cells. BEAS-2B cells were incubated with BCX (1.0, 2.0, 4.0 μmol/L) or MG624 (1.0 μmol/L) for 12 hours, or cells were serum starved and treated with compounds for 12 hours, followed by nicotine (1.0 μmol/L) or PNU (1.0 μmol/L) stimulation for 1 hour, or PDGF (100 ng/mL) or PMA (250 ng/mL) stimulation for 30 minutes. Then, cells were stained with TRITC-labeled phalloidin and analyzed using fluorescent microscopy. The representative fluorescent images are shown (A, the lamellipodia are indicated by arrowheads). The number of cells with significant lamellipodia was counted randomly in five fields (B). Scratch wound was generated in BEAS-2B cell monolayer, followed by treatment with BCX (0.5–4.0 μmol/L) or MG624 (1.0 μmol/L) in the presence or absence of PDGF or PMA stimulation for 12 hour. The width of wounded cell monolayer was measured in five random fields, and the inhibition was calculated respectively and compared with blank without stimulation (C).
Anita R. Iskandar et al. Cancer Prev Res 2016;9:
©2016 by American Association for Cancer Research