1. Fig. 1 Neu1−/− fibroblasts have characteristics of myofibroblasts.
Neu1−/− fibroblasts have characteristics of myofibroblasts. (A) Coronal gastrocnemius muscle sections of WT and Neu1−/− mice were stained with Masson’s trichrome, demonstrating massive collagen deposition (blue) in the Neu1−/− muscle. Scale bars, 50 μm. (B and C) FACS analysis of muscle connective tissue fibroblasts (CD45−/CD31−/αSMA−/TCF4+, n = 5) (B) and myofibroblasts (CD45−/CD31−/αSMA+/TCF4+, n = 3) (C) isolated from WT and Neu1−/− skeletal muscle at 3 to 4 months of age. (D and E) FACS analysis of MPs (CD45−/CD31−/Sca1−/integrin-α7+, n = 3) (D), and FAPs (CD45−/CD31−/Sca1+/integrin-α7−, n = 4) (E) isolated from WT and Neu1−/− skeletal muscle at 2 and 4.5 months of age. (F) Cell proliferation assayed in WT and Neu1−/− (myo)fibroblasts (n = 6). (G) Representative images of the invasion/migration assays performed with WT and Neu1−/− (myo)fibroblasts. Scale bar, 200 μm. (H) Quantification of (G) (n = 3). (I) Representative images of ex vivo invasion/migration of WT and Neu1−/− (myo)fibroblasts using ex vivo cultured peritoneal membranes. Scale bar, 100 μm. (J) Quantification of (I) (n = 4). Values are expressed as means ± SD. Statistical analysis was performed using the Student t test; *P < 0.05, ***P < 0.001, ****P <
Diantha van de Vlekkert et al. Sci Adv 2019;5:eaav3270
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