1. A Role for Regulatory T Cells in Cutaneous T-Cell Lymphoma; Induction of a CD4+CD25+Foxp3+ T-Cell Phenotype Associated with HTLV-1 Infection 
Patrick T. Walsh, Bernice M. Benoit, Maria Wysocka, Nicole M. Dalton, Laurence A. Turka, Alain H. Rook 
Journal of Investigative Dermatology 
Volume 126, Issue 3, Pages (March 2006)
DOI: /sj.jid
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2. Figure 1
Expression of Foxp3 in PBMCs from normal and CTCL patients. RNA from both patient (n=15) and normal PBMC (n=3) samples were screened for Foxp3 expression by quantitative real-time RT-PCR. Level of Foxp3 was measured relative to levels of β-actin. Primers were obtained as TaqMan Gene expression assays with the assay numbers Hs _m1 (FoxP3) and Hs _m1 (β-actin) (PE Applied Biosystems, Foster City, CA). Foxp3 expression in normal samples was taken as baseline expression. Results represent mean±standard deviation level of Foxp3 expression in individual samples.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Induction of a regulatory T-cell phenotype by HTLV-1 infection. (a) Percentage expression of CD25 on CD4+ T cells after coculture of CD4+CD25- T cells with either irradiated MJ cells or irradiated Jurkat cells for 8 weeks (n=4). Results expressed as mean±standard deviation. (b) Representative flow-cytometric analysis from (a) of induction of CD25 expression after 8-week coculture with irradiated MJ cells. (c) Quantitative real-time PCR analysis of Foxp3 expression on CD4+ T cells after coculture with the irradiated MJ cell line as in (a) above. Results depict mean±standard deviation expression level of Foxp3 relative to β-actin under each condition. Data are representative of four independent experiments with similar results. (d) TGF-β secretion by CD4+ T cells after coculture with irradiated MJ cells compared to TGF-β secreted by CD4+ T cells cocultured with the non-HTLV-1-positive jurkat cell line and cultures containing both irradiated cell lines alone. Results represent mean±standard deviation secretion from four independent coculture experiments. (e) Presence of HTLV-1 virus in CD4+ T cells after in-vitro coculture with irradiated MJ cell line detected by PCR of viral genome. Nonirradiated HTLV-1-positive (MJ) and -negative (Jurkat) cell lines used as controls for PCR.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions