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Volume 5, Issue 2, Pages (March 2012)

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1 Volume 5, Issue 2, Pages 418-429 (March 2012)
Drought Responses of Leaf Tissues from Wheat Cultivars of Differing Drought Tolerance at the Metabolite Level  Jairus B. Bowne, Tim A. Erwin, Juan Juttner, Thorsten Schnurbusch, Peter Langridge, Antony Bacic, Ute Roessner  Molecular Plant  Volume 5, Issue 2, Pages (March 2012) DOI: /mp/ssr114 Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

2 Figure 1 Relative Water Contents at Each Time Point for Each of the Three Cultivars. No measurement was taken at time point E for the control samples. Time point B samples of Kukri were missing from the experiment. Time points C and D in the drought samples were at the wilting point of Kukri. Excalibur follows the decline in RWC that Kukri does over time points A–C, but behaves more like RAC875 at time point D. Molecular Plant 2012 5, DOI: ( /mp/ssr114) Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

3 Figure 2 Metabolic Pathway Map for Kukri.
Control samples are represented in blue, drought-stressed samples in red. Metabolite plots with a green border are discussed in the ‘Results’ and ‘Discussion’ sections. Compounds with a blue border are metabolites that showed significant but minor (< fourfold) changes. Molecular Plant 2012 5, DOI: ( /mp/ssr114) Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

4 Figure 3 Metabolic Pathway Map for Excalibur.
Control samples are represented in blue, drought-stressed samples in red. Metabolite plots with a green border are discussed in the ‘Results’ and ‘Discussion’ sections. Compounds with a blue border are metabolites that showed significant but minor (< fourfold) decreases at time point C, similar to the changes seen in RAC875 at this time point. Molecular Plant 2012 5, DOI: ( /mp/ssr114) Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

5 Figure 4 Metabolic Pathway Map for RAC875.
Control samples are represented in blue, drought-stressed samples in red. Metabolites with a green border are specifically referred to in the ‘Results’ section. Compounds with a blue border are metabolites that showed significant but minor (< fourfold) decreases at time points C and E. Most of these compounds behaved in a similar manner to Excalibur at time point C but not at time point E. Molecular Plant 2012 5, DOI: ( /mp/ssr114) Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

6 Figure 5 Design of the Drought Stress Experiment.
Three time points were measured over the initial reduction in soil water availability to the wilting point of the most susceptible cultivar (Kukri) at time point C. D reflects the second wilting point for Kukri, and time point E was taken 24 h after the re-watering at time point D. Watering to weight is indicated by dots. Molecular Plant 2012 5, DOI: ( /mp/ssr114) Copyright © 2012 The Authors. All rights reserved. Terms and Conditions


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