1. Effect of incubating human sperm at room temperature on capacitation-related events 
Clara I. Marı́n-Briggiler, Ph.D., Jorge G. Tezón, Ph.D., Patricia V. Miranda, Ph.D., Mónica H. Vazquez-Levin, Ph.D. 
Fertility and Sterility 
Volume 77, Issue 2, Pages (February 2002)
DOI: /S (01)02982-X

2. FIGURE 1
Protein tyrosine phosphorylation patterns in human spermatozoa incubated at 37°C vs. 20°C. Motile spermatozoa were resuspended in HSM and incubated for 0 hours (lane A) and 18 hours at 37°C (lane B) or at 20°C (lane C). Sperm protein patterns were analyzed by Western immunoblot using a monoclonal antiphosphotyrosine antibody. Molecular weight standards (Mr × 10−3) are indicated on the left. A typical experiment is shown. This experiment was performed three times obtaining similar results.
Marı́n-Briggiler. Temperature and human sperm capacitation. Fertil Steril 2002.
Fertility and Sterility  , DOI: ( /S (01)02982-X)

3. FIGURE 2
Acrosome reaction in human spermatozoa incubated at 37°C vs. 20°C. After 18-hours incubation in HSM at 37°C (left panel) or at 20°C (right panel), spermatozoa were exposed to 10% human follicular fluid (hFF) or buffer (spontaneous; Spt) for 45 minutes at the same temperature. The percentage of acrosome-reacted spermatozoa was determined by Pisum sativum agglutinin staining. Results are expressed as mean ± SE, n = 15. aP<.001 vs. spontaneous AR at 37°C. bP<.001 vs. hFF AR at 37°C.
Marı́n-Briggiler. Temperature and human sperm capacitation. Fertil Steril 2002.
Fertility and Sterility  , DOI: ( /S (01)02982-X)

4. FIGURE 3
Inducibility of AR in human spermatozoa incubated under different temperature conditions. Motile spermatozoa were maintained for 18 hours in HSM at 37°C or 20°C and exposed to 10% human follicular fluid (hFF-induced AR) or buffer (spontaneous AR) at 37°C or 20°C. Four experimental conditions were then obtained, as indicated beneath the bars. AR inducibility was calculated as % hFF-induced AR minus % spontaneous AR. Results are expressed as mean ± SE, n = 15. aP<.001 vs. 37/37 and 37/20.
Marı́n-Briggiler. Temperature and human sperm capacitation. Fertil Steril 2002.
Fertility and Sterility  , DOI: ( /S (01)02982-X)

5. FIGURE 4
Protein tyrosine phosphorylation patterns in human spermatozoa subjected to variations in the incubation temperature. Motile spermatozoa were resuspended in HSM and incubated for 0 hours (lane A), for 18 hours at 37°C (lane B), for 18 hours at 20°C (lane C), for a total period of 18 hours with combined incubations at 37°C and 20°C (lanes D to G), for 16 hours at 37°C (lane H), or for 16 hours at 20°C (lane I). Sperm protein patterns were analyzed by Western immunoblot using a monoclonal antiphosphotyrosine antibody. Molecular weight standards (Mr × 10−3) are indicated on the left. A typical experiment is shown. This experiment was performed four times obtaining similar results.
Marı́n-Briggiler. Temperature and human sperm capacitation. Fertil Steril 2002.
Fertility and Sterility  , DOI: ( /S (01)02982-X)

6. FIGURE 5
Inducibility of AR in human spermatozoa subjected to variations in the incubation temperature. Motile spermatozoa were incubated for a total period of 18 hours in HSM as indicated beneath the bars and exposed to 10% human follicular fluid (hFF-induced AR) or buffer (spontaneous AR) at 37°C. The AR inducibility was calculated as % hFF-induced AR minus % spontaneous AR. Results are expressed as mean ± SE, n = 7. aP<.001 vs. 18 hours 37°C and 2 hours 20°C + 16 hours 37°C.
Marı́n-Briggiler. Temperature and human sperm capacitation. Fertil Steril 2002.
Fertility and Sterility  , DOI: ( /S (01)02982-X)