1. Impairments of NMDA receptor-dependent hippocampal synaptic plasticity in DGCs in GirdinSA/SA and Girdin+/− mice.
Impairments of NMDA receptor-dependent hippocampal synaptic plasticity in DGCs in GirdinSA/SA and Girdin+/− mice. A, B, Normal input–output curve at hippocampal MPP-DG synapses in GirdinSA/SA (A) and Girdin+/− (B) mice. Data are expressed as mean ± SEM (n = 10 for Girdin+/+, n = 10 for GirdinSA/SA in A; n = 10 for Girdin+/+, n = 10 for Girdin+/− in B). C, D, Normal paired-pulse ratio in GirdinSA/SA (C) and Girdin+/− (D) mice. Data are expressed as mean ± SEM (n = 9 for Girdin+/+, n = 9 for GirdinSA/SA in C; n = 9 for Girdin+/+, n = 9 for Girdin+/− in D). E–H, Impaired LTP in GirdinSA/SA (E, F) and Girdin+/− (G, H) mice. LTP was induced by TET (four trains of 100 Hz), and the fEPSP slope was recorded for 60 min after the TET. E, G, The LTP in all genotypes of mice was completely abolished by the NMDA receptor antagonist AP5 (100 μm). F, H, Quantification of the fold changes in the average fEPSP slope 0–5, 25–30, and 55–60 min after TET. Data are reported as mean ± SEM (n = 4–5 for Girdin+/+, n = 4–5 for GirdinSA/SA in E, F; n = 4–6 for Girdin+/+, n = 4–6 for Girdin+/− in G, H. *p < 0.05, **p < 0.01 compared with Girdin+/+ mice (Student's t test). I, K, Typical recordings of the AMPA and NMDA receptor-mediated EPSCs in GirdinSA/SA (I) and Girdin+/− (K) mice. J, L, Reduced NMDA/AMPA ratio in the DGCs in GirdinSA/SA (J) and Girdin+/− (L) mice. Data are expressed as mean ± SEM (n = 8 for Girdin+/+, n = 7 for GirdinSA/SA in J; n = 8 for Girdin+/+, n = 7 for Girdin+/− in L). **p < 0.01 compared with Girdin+/+ mice (Student's t test). M, N, Normal decay time constant of NMDA receptor-mediated EPSCs in the DGCs in GirdinSA/SA (M) and Girdin+/− (N) mice. Data are expressed as mean ± SEM (n = 8 for Girdin+/+, n = 7 for GirdinSA/SA in M; n = 8 for Girdin+/+, n = 7 for Girdin+/− in N). Electrophysiological recording was performed in the DG of 8- to 12-week-old mouse hippocampal slices. n indicates the number of slices tested.
Tsuyoshi Nakai et al. J. Neurosci. 2014;34:
©2014 by Society for Neuroscience