1. Ultraviolet B Radiation Upregulates the Production of Macrophage Migration Inhibitory Factor (MIF) in Human Epidermal Keratinocytes 
Tadamichi Shimizu, Riichiro Abe, Akira Ohkawara 
Journal of Investigative Dermatology 
Volume 112, Issue 2, Pages (February 1999)
DOI: /j x
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Concentration-dependent effects of UVB radiation on MIF and TNF-α production by cultured human keratinocytes. Keratinocytes were treated with UVB radiation and cultured for 48 h. MIF (A) and TNF-α (B) in culture media were measured by ELISA as described in Materials and Methods. The values are the mean ± SD of three different experiments.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
The time-course study of MIF and TNF-α production by cultured human keratinocytes by UVB irradiation. The culture supernatants were collected at different times after stimulation by UVB irradiation (50 mJ per cm2) and tested for MIF and TNF-α production. (A) MIF contents with (●) and without (○) UVB. (B) TNF-α contents with (▪) and without (□) UVB. Results are expressed as mean ± SD of three different experiments.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Epiderm, a human three-dimensional epidermal model, was exposed to 50 mJ per cm2 UVB irradiation. The culture supernatants were obtained before and 1, 6, 12, 24, and 48 h after UVB irradiation and their MIF contents were measured by ELISA as described in Materials and Methods. MIF contents with (●) and without (○) UVB irradiation. Results are expressed as mean ± SD of three different experiments.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Stimulation of MIF production by UVB irradiation derived from human epidermal sheets. Epidermal sheets were exposed to UVB light (50 mJ per cm2). After 12 or 24 h incubation, MIF content in the culture media was examined using MIF ELISA. The open and shaded bars show MIF contents without and with UVB irradiation, respectively. Results are expressed as mean ± SD of three different experiments.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Immunohistochemical analysis of MIF expression in human skin. Human skin biopsies in the medium were sham-irradiated (a), or UV-irradiated (50 mJ per cm2) (b), and incubated for 12 h. As a control to exclude nonspecific staining, nonimmune rabbit IgG was used as a control. Scale bar: 50 μm.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

7. Figure 6
MIF mRNA expression in cultured human keratinocytes. Total RNA was isolated from cultured human keratinocytes before and after UVB irradiation. Northern blot analysis was performed as described in Materials and Methods. The time-courses of MIF mRNA expression after UVB exposure of two different experiments (I and II) are shown.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

8. Figure 7
Serum MIF content after UVB irradiation. Five healthy volunteers were treated with a single total-body UVB exposure equivalent to four minimal erythema doses (240–280 mJ per cm2). Serum MIF levels were measured by MIF-specific ELISA as described in Materials and Methods before (□) and 48 h after UVB treatment (). Results are expressed as mean ± SD (n = 5).
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

9. Figure 8
The time-courses of serum MIF content after UVB exposure. Individual serum samples (a–e) were obtained before, and at various intervals after UVB exposure.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions