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Fig. 4 Super-resolution live imaging of calcium containing vesicle transports via lysosomes. Super-resolution live imaging of calcium containing vesicle.

Published byAnni-Kristiina Järvenpää Modified over 5 years ago

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Presentation on theme: "Fig. 4 Super-resolution live imaging of calcium containing vesicle transports via lysosomes. Super-resolution live imaging of calcium containing vesicle."— Presentation transcript:

1 Fig. 4 Super-resolution live imaging of calcium containing vesicle transports via lysosomes.
Super-resolution live imaging of calcium containing vesicle transports via lysosomes. (A) Snapshot of time-lapse SRM images of calcein-labeled osteoblasts. Cells were cultured with calcein and stained with Lysotracker and MitoTracker. White arrows indicate colocalization of lysosomes and calcein-positive vesicles. (B) Close-up of time-lapse SRM images of calcein-labeled osteoblasts. Red arrowheads indicate lysosome, and green arrowheads indicate calcein. Once lysosomes fused to calcein-positive vesicles adjacent to mitochondria, they started to move toward extracellular space. (C) Representative SRM image of LAMP1-mCherry–expressing cells. Cells were transfected with LAMP1-mCherry plasmid, cultured with calcein, and stained with MitoTracker. Calcein-positive vesicles matched to LAMP1-mCherry–positive lysosomes. (D) Schematic view of lysosomal involvement in transportation of calcium in mineralizing osteoblasts. Scale bars, 2 μm in (A), 1 μm in (B), and 10 μm in (C). Tomoaki Iwayama et al. Sci Adv 2019;5:eaax0672 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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