1. Histological analyses for limb-specific Uhrf1 KO mice.
Histological analyses for limb-specific Uhrf1 KO mice. (A) Alcian Blue and Nuclear Red staining of the proximal humerus at postnatal day 7; right panels show higher magnifications of the boxed areas in the left panels. The broken lines indicate the boundary between the growth plate zones. Representative data from at least three individual mice are shown. Scale bars: 100 μm. (B) The area and length of proliferative cartilage of control (n=3) and cKO (n=6) mice were measured for the region of interest (ROI) in the proximal humerus, which was separated into 10 equal parts per animal, using ImageJ. (C) Immunohistochemistry against type X collagen (Col10, green) for the proximal humerus at postnatal day 7. Representative data from at least three individual mice are shown. Scale bars: 200 µm. (D) The area and length of hypertrophic cartilage of control (n=4) and cKO (n=4) mice were measured, according to Col10-positive staining in C. All data are mean±s.d. *P<0.05 (Student's t-test or Mann–Whitney U-test).
Michiko Yamashita et al. Development 2018;145:dev157412
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