1. Lipid mass spectrometry analysis of WT, tgl3Δ and vps4Δ mutant cells.
Lipid mass spectrometry analysis of WT, tgl3Δ and vps4Δ mutant cells. (A–F) The lipid composition of WT, tgl3Δ and vps4Δ cells was analyzed in stationary phase and 5 h after dilution into fresh medium containing cerulenin. The indicated lipid species (TAG, triacylglycerols; DAG, diacylglycerol; PA, phosphatidic acid; PS, phosphatidylserine; PE, phosphatidylthanolamine; PC, phosphatidylcholine; PI, phosphatidylinositol) were analyzed by quantitative mass spectrometry. Data from two independent experiments were analyzed in duplicates and are shown as mean+s.d.
Sarah Ouahoud et al. J Cell Sci 2018;131:jcs213876
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