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AGE stimulation increases MMP2 and MMP9 secretion and activation in cultured retinal endothelial cells. AGE stimulation increases MMP2 and MMP9 secretion and activation in cultured retinal endothelial cells. Representative gelatin zymogram of the conditioned media collected from untreated cells (lane 1), cells treated with 50 μg/ml BSA (lane 2), and cells treated with 50 μg/ml AGE-BSA (Calbiochem) (lane 3). Following 48 h of AGE treatment, an increase in production of both the pro and activated forms of the enzymes was seen. Image represents different parts of the same gel. Deepti Navaratna et al. Diabetes 2007;56: ©2007 by American Diabetes Association
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